3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells

Autor: Zachary R. Gergely, Dana E. Martinez, Bryon S. Donohoe, Soren Mogelsvang, Rachel Herder, L. Andrew Staehelin

Publikováno v: Journal of Biological Research - Thessaloniki, Vol 25, Iss 1, Pp 1-16 (2018)

Abstract Background The insect-trapping leaves of Dionaea muscipula provide a model for studying the secretory pathway of an inducible plant secretory system. The leaf glands were induced with bovine serum albumin to secrete proteases that were chara

Externí odkaz: https://doaj.org/article/e54351a764014ef1b130442a7e37f625

Quantifying Yeast Microtubules and Spindles Using the Toolkit for Automated Microtubule Tracking (TAMiT)

Autor: Betterton, Saad Ansari, Zachary R. Gergely, Patrick Flynn, Gabriella Li, Jeffrey K. Moore, Meredith D.

Publikováno v: Biomolecules; Volume 13; Issue 6; Pages: 939

Fluorescently labeled proteins absorb and emit light, appearing as Gaussian spots in fluorescence imaging. When fluorescent tags are added to cytoskeletal polymers such as microtubules, a line of fluorescence and even non-linear structures results. W

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=multidiscipl::55734276e89a387e3ee20e74ea0cb418

The kinesin-5 protein Cut7 moves bidirectionally on fission yeast spindles with activity that increases in anaphase

Autor: Zachary R. Gergely, Saad Ansari, Michele H. Jones, Bojun Zhou, Cai Cash, Richard McIntosh, Meredith D. Betterton

Publikováno v: Journal of Cell Science. 136

Kinesin-5 motors are essential to separate mitotic spindle poles and assemble a bipolar spindle in many organisms. These motors crosslink and slide apart antiparallel microtubules via microtubule plus-end-directed motility. However, kinesin-5 localiz

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3942e5b50df3ed48b84fe94ee614d4a5
https://doi.org/10.1242/jcs.260474

Mechanisms of chromosome biorientation and bipolar spindle assembly analyzed by computational modeling

Autor: Saad Ansari, Meredith D. Betterton, Matthew A. Glaser, Christopher Edelmaier, J. Richard McIntosh, Adam Lamson, Zachary R. Gergely, Robert Blackwell

Publikováno v: eLife
eLife, Vol 9 (2020)

The essential functions required for mitotic spindle assembly and chromosome biorientation and segregation are not fully understood, despite extensive study. To illuminate the combinations of ingredients most important to align and segregate chromoso

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b32a5d12971f4bdd8c0ee9295effbe11
http://europepmc.org/articles/PMC7311174

3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells

Autor: Soren Mogelsvang, Bryon S. Donohoe, Dana Ethel Martinez, L. Andrew Staehelin, Zachary R. Gergely, Rachel Herder

Publikováno v: SEDICI (UNLP)
Universidad Nacional de La Plata
instacron:UNLP
Journal of Biological Research-Thessaloniki, Vol 25, Iss 1, Pp 1-16 (2018)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET

Background: The insect-trapping leaves of Dionaea muscipula provide a model for studying the secretory pathway of an inducible plant secretory system. The leaf glands were induced with bovine serum albumin to secrete proteases that were characterized

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f6d6a5e838b2e83749debf8994415b3
https://doi.org/10.1186/s40709-018-0086-2