Yeast cell death pathway requiring AP-3 vesicle trafficking leads to vacuole/lysosome membrane permeabilization

Autor: Zachary D. Stolp, Madhura Kulkarni, Yining Liu, Chengzhang Zhu, Alizay Jalisi, Si Lin, Arturo Casadevall, Kyle W. Cunningham, Fernando J. Pineda, Xinchen Teng, J. Marie Hardwick

Publikováno v: Cell reports. 39(2)

Unicellular eukaryotes have been suggested as undergoing self-inflicted destruction. However, molecular details are sparse compared with the mechanisms of programmed/regulated cell death known for human cells and animal models. Here, we report a mole

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6829049644ecd9834c0b065722fb0bcb
https://pubmed.ncbi.nlm.nih.gov/35417721

A Novel Two-Tag System for Monitoring Transport and Cleavage through the Classical Secretory Pathway - Adaptation to HIV Envelope Processing.

Autor: Zachary D Stolp, Aleksandr Stotland, Samantha Diaz, Brett J Hilton, Wesley Burford, Roland Wolkowicz

Publikováno v: PLoS ONE, Vol 8, Iss 6, p e68835 (2013)

The classical secretory pathway is essential for the transport of a host of proteins to the cell surface and/or extracellular matrix. While the pathway is well-established, many factors still remain to be elucidated. One of the most relevant biologic

Externí odkaz: https://doaj.org/article/3c58cec629dc47e3b1f909cabb8c99c4

A Multiplexed Cell-Based Assay for the Identification of Modulators of Pre-Membrane Processing as a Target against Dengue Virus

Autor: Zachary D. Stolp, Hakim Djaballah, Wesley Williams, Roland Wolkowicz, Andre Dharmawan, Cameron A. Smurthwaite, Connor W. Reed

Publikováno v: Journal of Biomolecular Screening

The DenV pre-membrane protein (prM) is a crucial chaperone for the viral envelope protein, preventing premature fusion with vesicles during viral export. prM molecules in immature particles are cleaved by host proteases, leading to mature fusogenic v

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a23639999e37d0208b7d015c3582426
http://europepmc.org/articles/PMC4438100

Whi2 is a conserved negative regulator of TORC1 in response to low amino acids

Autor: Ge He, Nicola L. Diny, Abdel Aouacheria, Margaret Dayhoff-Brannigan, Xianghui Chen, Cierra N. Sing, Zachary D. Stolp, Wen Chih Cheng, Mingjun Zhao, Kyle Metz, J. Marie Hardwick, Xinchen Teng, Guiqin Wang, Yu Zhang

Publikováno v: PLoS Genetics, Vol 14, Iss 8, p e1007592 (2018)
PLoS Genetics
PLoS Genetics, 2018, 14 (8), pp.e1007592. ⟨10.1371/journal.pgen.1007592⟩
PLoS Genetics, Public Library of Science, 2018, 14 (8), pp.e1007592. ⟨10.1371/journal.pgen.1007592⟩

International audience; Yeast WHI2 was originally identified in a genetic screen for regulators of cell cycle arrest and later suggested to function in general stress responses. However, the function of Whi2 is unknown. Whi2 has predicted structure a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c583efb63eb90e35e76b8524666ee49c
https://doi.org/10.1371/journal.pgen.1007592

Fluorescent genetic barcoding in mammalian cells for enhanced multiplexing capabilities in flow cytometry

Autor: Cameron A, Smurthwaite, Brett J, Hilton, Ryan, O'Hanlon, Zachary D, Stolp, Bryan M, Hancock, Darin, Abbadessa, Aleksandr, Stotland, Larry A, Sklar, Roland, Wolkowicz

Publikováno v: Cytometry. Part A : the journal of the International Society for Analytical Cytology. 85(1)

The discovery of the green fluorescent protein from Aequorea victoria has revolutionized the field of cell and molecular biology. Since its discovery a growing panel of fluorescent proteins, fluorophores and fluorescent-coupled staining methodologies

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::53db8865007b9de9bd3cb1364bfc1454
https://pubmed.ncbi.nlm.nih.gov/24591112