Scalable, Non-denaturing Purification of Phosphoproteins Using Ga³⁺-IMAC: N2A and M1M2 Titin Components as Study case

Autor: Adams, M, Fleming, JR, Riehle, E, Zhou, T, Zacharchenko, T, Markovic, M, Mayans, O

The purification of phosphorylated proteins in a folded state and in large enough quantity for biochemical or biophysical analysis remains a challenging task. Here, we develop a new implementation of the method of gallium immobilized metal chromatogr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=core_ac_uk__::f5d6624f7e7419fad0013dc9d86b8595
https://eprints.whiterose.ac.uk/145526/1/Gallium_ms_revised_1.pdf

Pressure-Dependent Chemical Shifts in the R3 Domain of Talin Show that It Is Thermodynamically Poised for Binding to Either Vinculin or RIAM

Autor: Baxter, N.J., Zacharchenko, T., Barsukov, I.L., Williamson, M.P.

Publikováno v: Baxter, N J, Zacharchenko, T, Barsukov, I L & Williamson, M P 2017, ' Pressure-Dependent Chemical Shifts in the R3 Domain of Talin Show that It Is Thermodynamically Poised for Binding to Either Vinculin or RIAM ', Structure, vol. 25, no. 12, pp. 1856-1866.e2 . https://doi.org/10.1016/j.str.2017.10.008
Structure

Talin mediates attachment of the cell to the extracellular matrix. It is targeted by the Rap1 effector RIAM to focal adhesion sites and subsequently undergoes force-induced conformational opening to recruit the actin-interacting protein vinculin. The

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https://www.research.manchester.ac.uk/portal/en/publications/pressuredependent-chemical-shifts-in-the-r3-domain-of-talin-show-that-it-is-thermodynamically-poised-for-binding-to-either-vinculin-or-riam(de886a46-28dd-4249-b91f-c30c17e27541).html

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LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex

Autor: Zacharchenko, T, Qian, X, Goult, BT, Jethwa, D, Almeida, TB, Ballestrem, C, Critchley, DR, Lowy, DR, Barsukov, IL

Publikováno v: Structure
Structure(London, England:1993)
Zacharchenko, T, Qian, X, Goult, BT T, Jethwa, D, Almeida, TB B, Ballestrem, C, Critchley, DR R, Lowy, DR R & Barsukov, IL L 2016, ' LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex ', Structure, vol. 24, no. 7, pp. 1130-1141 . https://doi.org/10.1016/j.str.2016.04.016

Summary Cell migration requires coordination between integrin-mediated cell adhesion to the extracellular matrix and force applied to adhesion sites. Talin plays a key role in coupling integrin receptors to the actomyosin contractile machinery, while

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http://livrepository.liverpool.ac.uk/3006862/2/1-s2.0-S0969212616300776-main.pdf

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