Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Zach Serber"'
Autor:
Zach Serber
Publikováno v:
Industrial Biotechnology. 15:325-327
Autor:
Jim Vallandingham, Harmen M. van Rossum, Kedar G. Patel, Shawn Szyjka, Zach Serber, Jed Dean, R. Cameron Coates, Stephen Blaskowski
Publikováno v:
Journal of Industrial Microbiology and Biotechnology. 46:187-201
This study details a reliable and efficient method for CRISPR–Cas9 genome engineering in the high amino acid-producing strain of Corynebacterium glutamicum, NRRL-B11474. Our investigation demonstrates that a plasmid-encoded single-guide RNA paired
Publikováno v:
Cell. 147:934-946
SummaryProtein phosphorylation provides a mechanism for the rapid, reversible control of protein function. Phosphorylation adds negative charge to amino acid side chains, and negatively charged amino acids (Asp/Glu) can sometimes mimic the phosphoryl
Autor:
Philipp Selenko, Gerhard Wagner, Zach Serber, Robert Hänsel, Volker Dötsch, Sina Reckel, Frank Löhr, James E. Ferrell
Publikováno v:
Nature Protocols. 1:2701-2709
The noninvasive character of NMR spectroscopy, combined with the sensitivity of the chemical shift, makes it ideally suited to investigate the conformation, binding events and dynamics of macromolecules inside living cells. These 'in-cell NMR' experi
Autor:
Sunil S. Chandran, Kedar G. Patel, Stefan de Kok, Jack D. Newman, Zach Serber, Leslie H. Stanton, Darren Platt, Victor Holmes, Maxime Durot, Todd Slaby, Jed Dean, Elaine B. Shapland
Publikováno v:
ACS synthetic biology. 3(2)
Assembly of DNA parts into DNA constructs is a foundational technology in the emerging field of synthetic biology. An efficient DNA assembly method is particularly important for high-throughput, automated DNA assembly in biofabrication facilities and
Autor:
Volker Dötsch, Zach Serber
Publikováno v:
Biochemistry. 40:14317-14323
The recent development of "in-cell NMR" techniques by two independent groups has demonstrated that NMR spectroscopy can be used to characterize the conformation and dynamics of biological macromolecules inside living cells. In this article, we descri
Publikováno v:
Journal of the American Chemical Society. 123:8895-8901
Our recently developed in-cell NMR procedure now enables one to observe protein conformations inside living cells. Optimization of the technique demonstrates that distinguishing the signals produced by a single protein species depends critically on p
Publikováno v:
Journal of Biomolecular NMR. 21:269-273
We describe a procedure that allows for very efficient identification of amino acid types in proteins by selective 15N-labeling. The usefulness of selective incorporation of 15N-labeled amino acids into proteins for the backbone assignment has been r
Determining proper responsiveness to incoming signals is fundamental to all biological systems. We demonstrate that intracellular signaling nodes can tune a signaling network's response threshold away from the basal median effective concentration est
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e4197ba55d9dd5591ffec055698ab87
https://europepmc.org/articles/PMC2880456/
https://europepmc.org/articles/PMC2880456/
We introduce a eukaryotic cellular system, the Xenopus laevis oocyte, for in-cell NMR analyses of biomolecules at high resolution and delineate the experimental reference conditions for successful implementations of in vivo NMR measurements in this c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93d3c33a1e4102e8bd934a590d696416
https://europepmc.org/articles/PMC1523310/
https://europepmc.org/articles/PMC1523310/