The hydrophobic core of twin-arginine signal sequences orchestrates specific binding to Tat-pathway related chaperones.

Autor: Anitha Shanmugham, Adil Bakayan, Petra Völler, Joost Grosveld, Holger Lill, Yves J M Bollen

Publikováno v: PLoS ONE, Vol 7, Iss 3, p e34159 (2012)

Redox enzyme maturation proteins (REMPs) bind pre-proteins destined for translocation across the bacterial cytoplasmic membrane via the twin-arginine translocation system and enable the enzymatic incorporation of complex cofactors. Most REMPs recogni

Externí odkaz: https://doaj.org/article/2f34ba0c2b9d4d27acc9d4b7cf247a21

Single-Molecule Imaging of Escherichia coli Transmembrane Proteins

Autor: Aravindan, Varadarajan, Felix, Oswald, Yves J M, Bollen

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1665

Single-molecule imaging in living cells can provide unique information about biological processes. Bacteria offer some particular challenges for single-molecule imaging due to their small size, only slightly larger than the diffraction limit of visib

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::43e28f8db41cf23468f276456d5e1008
https://pubmed.ncbi.nlm.nih.gov/28940067

The folding energy landscape of apoflavodoxin is rugged: Hydrogen exchange reveals nonproductive misfolded intermediates

Autor: Carlo P. M. van Mierlo, Monique B. Kamphuis, Yves J. M. Bollen

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America 103 (2006) 11
Proceedings of the National Academy of Sciences of the United States of America, 103(11), 4095-4100
Proceedings of the National Academy of Sciences of the United States of America, 103, 4095-4100. National Acad Sciences
Bollen, Y J M, Kamphuis, M B & Van Mierlo, C P M 2006, ' The folding energy landscape of apoflavodoxin is rugged: Hydrogen exchange reveals nonproductive misfolded intermediates. ', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, pp. 4095-4100 . https://doi.org/10.1073/pnas.0509133103

Many native proteins occasionally form partially unfolded forms (PUFs), which can be detected by hydrogen/deuterium exchange and NMR spectroscopy. Knowledge about these metastable states is required to better understand the onset of folding-related d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0179b28241d433ec79d8be1472d4abf2
https://doi.org/10.1073/pnas.0509133103

Formation of on- and off-pathway intermediates in the folding kinetics of azotobacter vinelandii apoflavodoxin

Autor: Yves J. M. Bollen, Ignacio E. Sánchez, Carlo P. M. van Mierlo

Publikováno v: Biochemistry 43 (2004) 32
Bollen, Y J M, Sanchez, I E & Van Mierlo, C P M 2004, ' Formation of on-and off-pathway intermediates in the folding kinetics of azotobacter vinelandii apoflavodoxin. ', Biochemistry, vol. 43, pp. 10475-10489 . https://doi.org/10.1021/bi049545m
Biochemistry, 43(32), 10475-10489
Biochemistry, 43, 10475-10489. American Chemical Society

The folding kinetics of the 179-residue Azotobacter vinelandii apoflavodoxin, which has an alpha-beta parallel topology, have been followed by stopped-flow experiments monitored by fluorescence intensity and anisotropy. Single-jump and interrupted re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbe4c046fe59a50006f3a9c201083bcf
https://hdl.handle.net/1871/29960

Fatal attraction in glycolysis: how Saccharomyces cerevisiae manages sudden transitions to high glucose

Autor: A. Wahl, Josephus Hulshof, Robert Planqué, J.H. van Heerden, Frank J. Bruggeman, Tom O'Toole, Joseph J. Heijnen, Meike T. Wortel, Bas Teusink, Yves J. M. Bollen

Publikováno v: van Heerden, J H, Wortel, M T, Bruggeman, F J, Heijnen, J J, Bollen, Y J M, Planque, R, Hulshof, J, O'Toole, T G, Wahl, A & Teusink, B 2014, ' Fatal attraction in glycolysis: how Saccharomyces cerevisiae manages sudden transitions to high glucose. ', Microbial Cell, vol. 1, no. 3, pp. 103-106 . https://doi.org/10.15698/mic2014.01.133
Microbial Cell, 1(3), 103-106. Shared Science Publishers
Microbial Cell, 1(3), 103-106. Shared Science Publishers OG
Microbial Cell, Vol 1, Iss 3, Pp 103-106 (2015)

In the model eukaryote Saccharomyces cerevisiae, it has long been known that a functional trehalose pathway is indispensable for transitions to high glucose conditions. Upon addition of glucose, cells with a defect in trehalose 6-phosphate synthase (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33753ed242fce662d35b084e558f3640
https://hdl.handle.net/1871.1/f69c6dab-b2e8-4751-9629-db311576c828