Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Yvan Chapdelaine"'
Publikováno v:
The Journal of Immunology. 171:5454-5460
The use of cytokines during vaccination, particularly IL-15, is being considered due to the unique ability of IL-15 to enhance the proliferation of memory CD8+ T cells. However, as homeostatic mechanisms limit excessive lymphocyte expansion, we addre
Publikováno v:
Journal of Virology. 76:11748-11752
The cauliflower mosaic virus (CaMV) capsid protein is derived by bidirectional processing of the precapsid protein (CP56). We expressed several derivatives of CP56 in Escherichia coli and used them as substrates for virus-associated kinase and casein
Autor:
Lakshmi Krishnan, Subash Sad, Henk van Faassen, Yvan Chapdelaine, Hao Shen, Renu Dudani, Dean K. Smith
Publikováno v:
The Journal of Immunology. 168:5737-5745
We evaluated CD8+ T cell responses against the dominant CTL epitope, OVA257–264, expressed by an acute (Listeria monocytogenes (LM) OVA) vs a chronic pathogen (Mycobacterium bovis bacillus Calmette-Guérin (BCG) OVA) to reveal the influence on CD8
Autor:
Dean K. Smith, Lakshmi Krishnan, Henk van Faassen, Yvan Chapdelaine, Renu Dudani, Hao Shen, Subash Sad
Publikováno v:
Infection and Immunity. 70:1957-1964
Induction of T-cell memory by vaccination ensures long-term protection against pathogens. We determined whether on-going inflammatory responses during vaccination influenced T-cell priming. A preexposure of mice toMycobacterium bovisBCG impaired thei
Autor:
Johanne Lemay, Rolf Morosoli, Donald Tremblay, Claude Dupont, Yvan Chapdelaine, Michel Gilbert
Publikováno v:
Canadian Journal of Microbiology. 48:43-48
Two major antigens from Mycobacterium tuberculosis were produced by Streptomyces lividans as secreted extracellular proteins. An expression-secretion vector had been constructed that contained the promoter of xylanase A and the signal sequence of cel
Autor:
Thomas Hohn, Yvan Chapdelaine
Publikováno v:
Virus Genes. 17:139-150
The capsid protein of the cauliflower mosaic virus (CaMV) was expressed in a bacterial system to study CaMV assembly. Bacterial lysates contained soluble particulate material and insoluble inclusion bodies that were both used for analysis. In vitro r
Publikováno v:
Virology. 217(1):147-157
The cauliflower mosaic virus (CaMV) inclusion body protein (pVI) is able to specifically interact with the viral capsid precursor protein (pIV). By using the yeast two-hybrid system and a blot assay, the pIV region required for the recognition of pVI
Autor:
Subash Sad, Yvan Chapdelaine, Henk van Faassen, Renu Dudani, Joao A. Pedras-Vasconcelos, Dean K. Smith
Publikováno v:
Journal of immunology (Baltimore, Md. : 1950). 169(3)
Induction and maintenance of T cell memory is critical for the control of intracellular pathogens and tumors. Memory T cells seem to require few “maintenance signals,” though often such studies are done in the absence of competing immune challeng
Autor:
Henk van Faassen, Yvan Chapdelaine, Subash Sad, Renu Dudani, Dean K. Smith, Joao A. Pedras-Vasconcelos
Publikováno v:
Infection and immunity. 70(8)
Type 1 T-cell responses against intracellular pathogens play a crucial role in mediating protection. We examined whether the induction of a strong type 1 T-cell response during a chronic bacterial infection influences responses to superantigens capab
Publikováno v:
Journal of virology. 73(1)
The entry of the viral genomic DNA of cauliflower mosaic virus into the nucleus is a critical step of viral infection. We have shown by transient expression in plant protoplasts that the viral coat protein (CP), which is processed from the product of