Zobrazeno 1 - 10
of 150
pro vyhledávání: '"Yutaka Hanazono"'
Publikováno v:
International Journal of Translational Medicine, Vol 4, Iss 3, Pp 570-583 (2024)
Imaging technologies are used to observe the morphology and function of various organs in the body and have become indispensable in a multitude of fields, ranging from basic research to clinical medicine. The luminescence technology based on the luci
Externí odkaz:
https://doaj.org/article/8e4417d4d14c421195eb4307f480e49e
Autor:
Misa Minegishi, Takahiro Kuchimaru, Kaori Nishikawa, Takayuki Isagawa, Satoshi Iwano, Kei Iida, Hiromasa Hara, Shizuka Miura, Marika Sato, Shigeaki Watanabe, Akifumi Shiomi, Yo Mabuchi, Hiroshi Hamana, Hiroyuki Kishi, Tatsuyuki Sato, Daigo Sawaki, Shigeru Sato, Yutaka Hanazono, Atsushi Suzuki, Takahide Kohro, Tetsuya Kadonosono, Tomomi Shimogori, Atsushi Miyawaki, Norihiko Takeda, Hirofumi Shintaku, Shinae Kizaka-Kondoh, Satoshi Nishimura
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Abstract Cancer cells inevitably interact with neighboring host tissue-resident cells during the process of metastatic colonization, establishing a metastatic niche to fuel their survival, growth, and invasion. However, the underlying mechanisms in t
Externí odkaz:
https://doaj.org/article/c373ff5e397541ef903bbdc92c8afb6e
PAM-flexible Cas9-mediated base editing of a hemophilia B mutation in induced pluripotent stem cells
Autor:
Takafumi Hiramoto, Yuji Kashiwakura, Morisada Hayakawa, Nemekhbayar Baatartsogt, Nobuhiko Kamoshita, Tomoyuki Abe, Hiroshi Inaba, Hiroshi Nishimasu, Hideki Uosaki, Yutaka Hanazono, Osamu Nureki, Tsukasa Ohmori
Publikováno v:
Communications Medicine, Vol 3, Iss 1, Pp 1-12 (2023)
Abstract Background Base editing via CRISPR-Cas9 has garnered attention as a method for correcting disease-specific mutations without causing double-strand breaks, thereby avoiding large deletions and translocations in the host chromosome. However, i
Externí odkaz:
https://doaj.org/article/8d87658f0b3149538b80ca60fb8ca67c
Autor:
Suvd Byambaa, Hideki Uosaki, Tsukasa Ohmori, Hiromasa Hara, Hitoshi Endo, Osamu Nureki, Yutaka Hanazono
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 20, Iss , Pp 451-462 (2021)
We conducted two lines of genome-editing experiments of mouse hematopoietic stem cells (HSCs) with the clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein 9 (Cas9). First, to evaluate the genome-editing eff
Externí odkaz:
https://doaj.org/article/2f08446944e44cac965321da03925c00
Autor:
Tsukasa Ohmori, Yasumitsu Nagao, Hiroaki Mizukami, Asuka Sakata, Shin-ichi Muramatsu, Keiya Ozawa, Shin-ichi Tominaga, Yutaka Hanazono, Satoshi Nishimura, Osamu Nureki, Yoichi Sakata
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Abstract Haemophilia B, a congenital haemorrhagic disease caused by mutations in coagulation factor IX gene (F9), is considered an appropriate target for genome editing technology. Here, we describe treatment strategies for haemophilia B mice using t
Externí odkaz:
https://doaj.org/article/b79acbb0c9004973b1526e004ab7e566
Autor:
Yoshikazu Arai, Hisho Fukukawa, Takanori Atozi, Shoma Matsumoto, Yutaka Hanazono, Hiroshi Nagashima, Jun Ohgane
Publikováno v:
PLoS ONE, Vol 11, Iss 1, p e0146498 (2016)
DNA methylation is an epigenetic modification important for cell fate determination and cell type-specific gene expression. Transcriptional regulatory regions of the mammalian genome contain a large number of tissue/cell type-dependent differentially
Externí odkaz:
https://doaj.org/article/87a5051794fa496bbb03022d98686bc3
Autor:
Masahito Watanabe, Kazuaki Nakano, Hitomi Matsunari, Taisuke Matsuda, Miki Maehara, Takahiro Kanai, Mirina Kobayashi, Yukina Matsumura, Rieko Sakai, Momoko Kuramoto, Gota Hayashida, Yoshinori Asano, Shuko Takayanagi, Yoshikazu Arai, Kazuhiro Umeyama, Masaki Nagaya, Yutaka Hanazono, Hiroshi Nagashima
Publikováno v:
PLoS ONE, Vol 9, Iss 1 (2014)
Externí odkaz:
https://doaj.org/article/a1bf68f0708c47849f2d20129a6a99a9
Autor:
Yoshihisa Mizukami, Tomoyuki Abe, Hiroaki Shibata, Yukitoshi Makimura, Shuh-hei Fujishiro, Kimihide Yanase, Shuji Hishikawa, Eiji Kobayashi, Yutaka Hanazono
Publikováno v:
PLoS ONE, Vol 9, Iss 6, p e98319 (2014)
Recent studies have revealed negligible immunogenicity of induced pluripotent stem (iPS) cells in syngeneic mice and in autologous monkeys. Therefore, human iPS cells would not elicit immune responses in the autologous setting. However, given that hu
Externí odkaz:
https://doaj.org/article/7f148136da7242de86b84d190120b97a
Generating porcine chimeras using inner cell mass cells and parthenogenetic preimplantation embryos.
Autor:
Kazuaki Nakano, Masahito Watanabe, Hitomi Matsunari, Taisuke Matsuda, Kasumi Honda, Miki Maehara, Takahiro Kanai, Gota Hayashida, Mirina Kobayashi, Momoko Kuramoto, Yoshikazu Arai, Kazuhiro Umeyama, Shuh-Hei Fujishiro, Yoshihisa Mizukami, Masaki Nagaya, Yutaka Hanazono, Hiroshi Nagashima
Publikováno v:
PLoS ONE, Vol 8, Iss 4, p e61900 (2013)
BACKGROUND: The development and validation of stem cell therapies using induced pluripotent stem (iPS) cells can be optimized through translational research using pigs as large animal models, because pigs have the closest characteristics to humans am
Externí odkaz:
https://doaj.org/article/bef48ecd755c47d981fb4697ab980e2a
Autor:
Masahito Watanabe, Kazuaki Nakano, Hitomi Matsunari, Taisuke Matsuda, Miki Maehara, Takahiro Kanai, Mirina Kobayashi, Yukina Matsumura, Rieko Sakai, Momoko Kuramoto, Gota Hayashida, Yoshinori Asano, Shuko Takayanagi, Yoshikazu Arai, Kazuhiro Umeyama, Masaki Nagaya, Yutaka Hanazono, Hiroshi Nagashima
Publikováno v:
PLoS ONE, Vol 8, Iss 10, p e76478 (2013)
Zinc finger nuclease (ZFN) is a powerful tool for genome editing. ZFN-encoding plasmid DNA expression systems have been recently employed for the generation of gene knockout (KO) pigs, although one major limitation of this technology is the use of po
Externí odkaz:
https://doaj.org/article/8b3107db5c99480d9579f29e3686a755