Zobrazeno 1 - 10
of 57
pro vyhledávání: '"Yusuke Kamachi"'
Publikováno v:
Cells, Vol 13, Iss 3, p 261 (2024)
Successful genome editing depends on the cleavage efficiency of programmable nucleases (PNs) such as the CRISPR–Cas system. Various methods have been developed to assess the efficiency of PNs, most of which estimate the occurrence of indels caused
Externí odkaz:
https://doaj.org/article/a57b3e954db7435189f9c60549af28d6
Autor:
Deshani C. Ranawakage, Keita Okada, Kota Sugio, Yuya Kawaguchi, Yuki Kuninobu-Bonkohara, Takuya Takada, Yusuke Kamachi
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish (Da
Externí odkaz:
https://doaj.org/article/0c8dc2dc68eb4a5ebc6159214f78efde
Publikováno v:
PLoS Genetics, Vol 6, Iss 5, p e1000936 (2010)
The B1 SOX transcription factors SOX1/2/3/19 have been implicated in various processes of early embryogenesis. However, their regulatory functions in stages from the blastula to early neurula remain largely unknown, primarily because loss-of-function
Externí odkaz:
https://doaj.org/article/21746bce46584fcb8e1cd84b01de4d3a
Autor:
Keita Okada, Kanae Aoki, Teruyuki Tabei, Kota Sugio, Katsunori Imai, Yuki Bonkohara, Yusuke Kamachi
Publikováno v:
Nucleic Acids Research. 50:2854-2871
Specific sequence features of the protospacer and protospacer-adjacent motif (PAM) are critical for efficient cleavage by CRISPR-Cas9, but current knowledge is largely derived from single-guide RNA (sgRNA) systems assessed in cultured cells. In this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2a5cf89db35c48aebf55408cfacaa8d
https://doi.org/10.1093/nar/gkac100
https://doi.org/10.1093/nar/gkac100
Autor:
Yusuke Kamachi, Atsuo Kawahara
Publikováno v:
Methods in Molecular Biology ISBN: 9781071630150
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b311bc595d05a5961636991adde7ceab
https://doi.org/10.1007/978-1-0716-3016-7_24
https://doi.org/10.1007/978-1-0716-3016-7_24
Autor:
Deshani C. Ranawakage, Takuya Takada, Yuki Kuninobu-Bonkohara, Yusuke Kamachi, Keita Okada, Yuya Kawaguchi, Kota Sugio
Publikováno v:
Frontiers in Cell and Developmental Biology
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Frontiers in Cell and Developmental Biology, Vol 8 (2021)
Despite the unprecedented gene editing capability of CRISPR-Cas9-mediated targeted knock-in, the efficiency and precision of this technology still require further optimization, particularly for multicellular model organisms, such as the zebrafish (Da
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c79710d470cd5a01fd666a2e43e4c8e9
https://pubmed.ncbi.nlm.nih.gov/33681181
https://pubmed.ncbi.nlm.nih.gov/33681181
Publikováno v:
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-16 (2019)
Scientific Reports, Vol 9, Iss 1, Pp 1-16 (2019)
The affinity of an antibody for its antigen serves as a critical parameter for antibody evaluation. The evaluation of antibody-antigen affinity is essential for a successful antibody-based assay, particularly immunoprecipitation (IP), due to its stri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41e1305694f177452a6d64ca8bb7c36d
https://pubmed.ncbi.nlm.nih.gov/31053795
https://pubmed.ncbi.nlm.nih.gov/31053795
Autor:
Masanori Uchikawa, Hisato Kondoh, Yu Okamoto, Naoko Nishimura, Deshani C. Ranawakage, Kazunari Matsuda, Yusuke Kamachi
Publikováno v:
Development, growthdifferentiation. 60(3)
To elucidate the transcriptional regulation that underlies specification of the otic placode, we investigated the Sox3 downstream enhancer Otic1 of the chicken, the activity of which is restricted to and distributed across the entire otic placode. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::328d2e876658a800df7d4917f054fb01
https://pubmed.ncbi.nlm.nih.gov/29520762
https://pubmed.ncbi.nlm.nih.gov/29520762
Autor:
Essam M. Abdelalim, Natacha A. Agabalyan, Parth Armin, Jessica Bertolini, Jeff Biernaskie, Ian Chambers, Kathryn S.E. Cheah, G. Marius Clore, Mohamed M. Emara, Rebecca Favaro, Andrew Hagner, Yasuo Ishii, Ian Jacobs, Ming Jiang, Yusuke Kamachi, Prasanna R. Kolatkar, Hisato Kondoh, Wei-Yao Ku, Robin Lovell-Badge, Jessica Mariani, Sara Mercurio, Balasubramanian Moovarkumudalvan, Jonas Muhr, Nicholas P. Mullin, Silvia K. Nicolis, Sergio Ottolenghi, Raymond A. Poot, Nilima Prakash, Jianwen Que, Waleed Rahmani, Karine Rizzoti, Masanori Uchikawa, Veronica van Heyningen, Frederick C.K. Wong, Neng Chun Wong, Pin-Xian Xu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::63e3ba0bfd18ec3297344d0e141a8adc
https://doi.org/10.1016/b978-0-12-800352-7.01002-1
https://doi.org/10.1016/b978-0-12-800352-7.01002-1
Autor:
Yusuke Kamachi
Sox2 ( SRY-box 2 ) is a member of the SoxB1 group of genes, which is composed of Sox1, Sox2 , and Sox3 in amniotes. In teleost fish, the SoxB1 group includes Sox19 , which is an ortholog of Sox15 , a sole member of the mammalian SoxG group. The Sox f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::88ae1a90e47bb903b0266c7e31d2578a
https://doi.org/10.1016/b978-0-12-800352-7.00006-2
https://doi.org/10.1016/b978-0-12-800352-7.00006-2