Výsledky vyhledávání - "Yusei Matsuzaki"

Akademický článek

Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies

Autor: Yusei Matsuzaki, Wataru Aoki, Takumi Miyazaki, Shunsuke Aburaya, Yuta Ohtani, Kaho Kajiwara, Naoki Koike, Hiroyoshi Minakuchi, Natsuko Miura, Tetsuya Kadonosono, Mitsuyoshi Ueda

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)

Abstract Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed pep

Externí odkaz: https://doaj.org/article/6ca696081ad148fc8e2a41f6d690936f

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Akademický článek

Peptide barcoding for establishment of new types of genotype-phenotype linkages.

Autor: Kana Miyamoto, Wataru Aoki, Yuta Ohtani, Natsuko Miura, Shunsuke Aburaya, Yusei Matsuzaki, Kaho Kajiwara, Yoshinori Kitagawa, Mitsuyoshi Ueda

Publikováno v: PLoS ONE, Vol 14, Iss 4, p e0215993 (2019)

Measuring binding properties of binders (e.g., antibodies) is essential for developing useful experimental reagents, diagnostics, and pharmaceuticals. Display technologies can evaluate a large number of binders in a high-throughput manner, but the im

Externí odkaz: https://doaj.org/article/c0de412af4f444c3a965f543cc618835

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The lysosomal Ragulator complex activates NLRP3 inflammasome in vivo via HDAC6

The cellular activation of the NLRP3 inflammasome is spatiotemporally orchestrated by various organelles, but whether lysosomes contribute to this process remains unclear. Here, we show the vital role of the lysosomal membrane-tethered Ragulator comp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04657f2a72b81ed0d935697098ec1e17
https://hdl.handle.net/11588/906504

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Production of Single-Domain Antibodies in Pichia pastoris

Autor: Yusei, Matsuzaki, Kaho, Kajiwara, Wataru, Aoki, Mitsuyoshi, Ueda

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2446

Single-domain antibodies (sdAbs) are binders that consist of a single immunoglobulin domain. SdAbs have gained importance as therapeutics, diagnostic reagents, and research tools. Functional sdAbs are commonly produced in Escherichia coli, which is a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e710ce053284e5fdea75d1115ed56127
https://pubmed.ncbi.nlm.nih.gov/35157274

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Production of Single-Domain Antibodies in Pichia pastoris

Autor: Yusei Matsuzaki, Kaho Kajiwara, Wataru Aoki, Mitsuyoshi Ueda

Publikováno v: Methods in Molecular Biology ISBN: 9781071620748

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1b9dcefdef003cb00e7e6d5337fd920f
https://doi.org/10.1007/978-1-0716-2075-5_9

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Peptide barcoding for one-pot evaluation of sequence-function relationships of nanobodies

Autor: Shunsuke Aburaya, Yuta Ohtani, Wataru Aoki, Takumi Miyazaki, Naoki Koike, Yusei Matsuzaki, Hiroyoshi Minakuchi, Tetsuya Kadonosono, Mitsuyoshi Ueda, Kaho Kajiwara, Natsuko Miura

Publikováno v: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)

Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed peptide barc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c72e48ce77db53e4d4c54ccc90ec0be9
https://pubmed.ncbi.nlm.nih.gov/34728738

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