In situ proteolysis for protein crystallization and structure determination

Autor: Maria Dolores Herman, Masoud Vedadi, Jinrong Min, Slav Dimov, Hui Ouyang, Ida Johansson, Aiping Dong, Haizhong Zhu, Rosa Di Leo, Tatiana Skarina, Susanne Flodin, Alexei Savchenko, Ting Xiao, Changsoo Chang, Raymond Hui, Aled M. Edwards, Hee-Won Park, Masato Akutsu, George V. Avvakumov, Elena Evdokimova, Martin Welin, P. Nordlund, Maksymilian Chruszcz, Natalie R Klostermann, Yufeng Tong, Cheryl H. Arrowsmith, Ekaterina V. Filippova, Wolfram Tempel, Lars Göran Dahlgren, Andrzej Joachimiak, Marianne Cuff, Ludmila Dombrovski, Qiuni Que, Olga Egorova, Tomas Nyman, Xiaohui Xu, Jennifer L. Guthrie, Alexander F. Yakunin, Rongguang Zhang, Adelinda Yee, Alexandr Ignatchenko, Jun Gu, John R. Walker, Johan Weigelt, Hong Zeng, Yang Shen, Wael M. Rabeh, Sirano Dhe-Paganon, Kemin Tan, Yongson Liu, Alexey Bochkarev, Wladek Minor, Deepthi Sukumard, Lionel Tresagues, Hong Wu, Youngchang Kim, Hong Zheng, Bum Soo Hong, Martina Nilsson, Marcin Cymborowski, Chao Qi, P.J. Finerty, Veronica Yim, Martin Hammerström, Limin Shen, Yuri Korniyenko, Lyudmila Nedyalkova, A. Flores, Susanne Gräslund

Publikováno v: Nature Methods. 4:1019-1021

We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins tha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d384f298d521962cc871f139ce3a129a
https://doi.org/10.1038/nmeth1118