Protocol for transcriptome-wide mapping of small-molecule RNA-binding sites in live cells

Autor: Yuquan Tong, Patrick R.A. Zanon, Xueyi Yang, Xiaoxuan Su, Jessica L. Childs-Disney, Matthew D. Disney

Publikováno v: STAR Protocols, Vol 5, Iss 3, Pp 103271- (2024)

Summary: Small molecules targeting RNA can be valuable chemical probes and potential therapeutics. The interactions between small molecules, particularly fragments, and RNA, however, can be difficult to detect due to their modest affinities and short

Externí odkaz: https://doaj.org/article/bf84e12dc49843aab28fe12b74c17a66

Programming inactive RNA-binding small molecules into bioactive degraders

Autor: Yuquan Tong, Yeongju Lee, Xiaohui Liu, Jessica L. Childs-Disney, Blessy M. Suresh, Raphael I. Benhamou, Chunying Yang, Weimin Li, Matthew G. Costales, Hafeez S. Haniff, Sonja Sievers, Daniel Abegg, Tristan Wegner, Tiffany O. Paulisch, Elizabeth Lekah, Maison Grefe, Gogce Crynen, Montina Van Meter, Tenghui Wang, Quentin M. R. Gibaut, John L. Cleveland, Alexander Adibekian, Frank Glorius, Herbert Waldmann, Matthew D. Disney

Publikováno v: Nature. 618:169-179

Target occupancy is often insufficient to elicit biological activity, particularly for RNA, compounded by the longstanding challenges surrounding the molecular recognition of RNA structures by small molecules. Here we studied molecular recognition pa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8ddd01fdc061bbc5774956f8b2b5aaea
https://doi.org/10.1038/s41586-023-06091-8

Targeting RNA structures with small molecules

Autor: Jessica L. Childs-Disney, Xueyi Yang, Quentin M. R. Gibaut, Yuquan Tong, Robert T. Batey, Matthew D. Disney

Publikováno v: Nature Reviews Drug Discovery. 21:736-762

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::938dece0be80ea56341e14ccddcdadd7
https://doi.org/10.1038/s41573-022-00521-4

Bioinformatic Searching for Optimal RNA Targets of Dimeric Compounds Informs Design of a MicroRNA-27a Inhibitor

Autor: Raphael I. Benhamou, Shruti Choudhary, Elizabeth Lekah, Yuquan Tong, Matthew D. Disney

Publikováno v: ACS Chem Biol

Various studies have shown that selective molecular recognition of RNA targets by small molecules in cells, although challenging, is indeed possible. One facile strategy to enhance selectivity and potency is binding two or more sites within an RNA si

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12165a5b28f1b57c4a8bbb5e81a56ecc
https://pubmed.ncbi.nlm.nih.gov/34898169

A blood-brain penetrant RNA-targeted small molecule triggers elimination of r(G4C2)exp in c9ALS/FTD via the nuclear RNA exosome

Autor: Jessica A. Bush, Samantha M. Meyer, Rita Fuerst, Yuquan Tong, Yue Li, Raphael I. Benhamou, Haruo Aikawa, Patrick R. A. Zanon, Quentin M. R. Gibaut, Alicia J. Angelbello, Tania F. Gendron, Yong-Jie Zhang, Leonard Petrucelli, Torben Heick Jensen, Jessica L. Childs-Disney, Matthew D. Disney

Publikováno v: Bush, J A, Meyer, S M, Fuerst, R, Tong, Y, Li, Y, Benhamou, R I, Aikawa, H, Zanon, P R A, Gibaut, Q M R, Angelbello, A J, Gendron, T F, Zhang, Y J, Petrucelli, L, Heick Jensen, T, Childs-Disney, J L & Disney, M D 2022, ' A blood-brain penetrant RNA-targeted small molecule triggers elimination of r(G 4 C 2 ) exp in c9ALS/FTD via the nuclear RNA exosome ', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 48, e2210532119 . https://doi.org/10.1073/pnas.2210532119

A hexanucleotide repeat expansion in intron 1 of the C9orf72 gene is the most common genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia, or c9ALS/FTD. The RNA transcribed from the expansion, r(G 4 C 2 ) exp , causes various pa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d99afbe6d3582820279ae53936a1dca4
https://pure.au.dk/portal/da/publications/a-bloodbrain-penetrant-rnatargeted-small-molecule-triggers-elimination-of-rg4c2exp-in-c9alsftd-via-the-nuclear-rna-exosome(c64b1cee-2f4b-4d36-8329-562cc33c1c4c).html