Stochastic priming and spatial cues orchestrate heterogeneous clonal contribution to mouse pancreas organogenesis

Autor: Hjalte List Larsen, Laura Martín-Coll, Alexander Valentin Nielsen, Christopher V. E. Wright, Ala Trusina, Yung Hae Kim, Anne Grapin-Botton

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-13 (2017)

The pancreas arises from a small population of cells but how individual cells contribute to organ formation is unclear. Here, the authors deconstruct pancreas organogenesis into clonal units, showing that single progenitors give rise to heterogeneous

Externí odkaz: https://doaj.org/article/5617ac1576cd4daf93bca36ae542b223

Pancreas organoid models of development and regeneration

Autor: Anne Grapin-Botton, Yung Hae Kim

Publikováno v: Development (Cambridge, England). 149(20)

Organoids have become one of the fastest progressing and applied models in biological and medical research, and various organoids have now been developed for most of the organs of the body. Here, we review the methods developed to generate pancreas o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac696ada079b3f2b6c8405df35aa456f
https://pubmed.ncbi.nlm.nih.gov/36314540

Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation

Autor: Akiko Nakamura, Yan Fung Wong, Andrea Venturato, Magali Michaut, Seshasailam Venkateswaran, Mithun Santra, Carla Gonçalves, Michael Larsen, Marit Leuschner, Yung Hae Kim, Joshua Brickman, Mark Bradley, Anne Grapin-Botton

Publikováno v: Nakamura, A, Wong, Y F, Venturato, A, Michaut, M, Venkateswaran, S, Santra, M, Gonçalves, C, Larsen, M, Leuschner, M, Kim, Y H, Brickman, J, Bradley, M & Grapin-Botton, A 2022, ' Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation ', Stem Cell Reports, vol. 17, no. 5, pp. 1215-1228 . https://doi.org/10.1016/j.stemcr.2022.03.013
Stem Cell Rep. 17, 1215-1228 (2022)

With the aim of producing β cells for replacement therapies to treat diabetes, several protocols have been developed to differentiate human pluripotent stem cells to β cells via pancreatic progenitors. While invivo pancreatic progenitors expand thr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e68e57a41f1d86c7de5f730f38f01101
https://curis.ku.dk/ws/files/311215411/1_s2.0_S2213671122001461_main.pdf

A 3D system to model human pancreas development and its reference single-cell transcriptome atlas identify signaling pathways required for progenitor expansion

Autor: Yung Hae Kim, Anne Grapin-Botton, Rashmiparvathi Keshara, Signe Perlman, Sascha Jung, Antonio del Sol, Michael Larsen, Carla A C Gonçalves, Akiko Nakamura, Kristine J. Hare, Lene Lundvall, Lena Hersemann, Johannes Stratmann, Ido Amit, Lea Langhoff Thuesen, Anne Jørgensen, Marit Leuschner

Publikováno v: Goncalves, C A, Larsen, M, Jung, S, Stratmann, J, Nakamura, A, Leuschner, M, Hersemann, L, Keshara, R, Perlman, S, Lundvall, L, Thuesen, L L, Hare, K J, Amit, I, Jørgensen, A, Kim, Y H, del Sol, A & Grapin-Botton, A 2021, ' A 3D system to model human pancreas development and its reference single-cell transcriptome atlas identify signaling pathways required for progenitor expansion ', Nature Communications, vol. 12, 3144 . https://doi.org/10.1038/s41467-021-23295-6
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)

Human organogenesis remains relatively unexplored for ethical and practical reasons. Here, we report the establishment of a single-cell transcriptome atlas of the human fetal pancreas between 7 and 10 post-conceptional weeks of development. To interr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::015b3dc2a4cab00c0944a7f613c56104
https://doi.org/10.21203/rs.3.pex-1430/v1

Self-organization of organoids from endoderm-derived cells

Autor: Anne Grapin-Botton, Allison Lewis, Yung Hae Kim, Rashmiparvathi Keshara

Publikováno v: Lewis, A, Keshara, R, Kim, Y H & Grapin-Botton, A 2021, ' Self-organization of organoids from endoderm-derived cells ', Journal of Molecular Medicine, vol. 99, pp. 449-462 . https://doi.org/10.1007/s00109-020-02010-w
Journal of Molecular Medicine (Berlin, Germany)

Organoids constitute biological systems which are used to model organ development, homeostasis, regeneration, and disease in vitro and hold promise for use in therapy. Reflecting in vivo development, organoids form from tissue cells or pluripotent st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af2bb4fdc9bb3d040b02e0813e0b7cf2
https://curis.ku.dk/portal/da/publications/selforganization-of-organoids-from-endodermderived-cells(d89af857-8abe-4c7a-bd4f-3ca99f673954).html