Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From Pseudomonas fluorescens Immobilized on Octyl-Agarose Beads

Autor: Carmen Mendez-Sanchez, Yuliya Lokha, Diego Carballares, Sara Arana-Peña, Roberto Fernandez-Lafuente, Luciana Rocha Barros Gonçalves, Nathalia Saraiva Rios

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Repositório Institucional da UFRN
Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN

The lipase from Pseudomonas fluorescens (PFL) has been immobilized on octyl-agarose beads under 16 different conditions (varying pH, ionic strength, buffer, adding some additives) at two different loadings, 1 and 60 mg of enzyme/g of support with the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09ab4ac0cd9d8f46885a7035894272bf
https://www.frontiersin.org/article/10.3389/fbioe.2020.00036/full

Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From

Autor: Sara, Arana-Peña, Nathalia S, Rios, Diego, Carballares, Carmen, Mendez-Sanchez, Yuliya, Lokha, Luciana R B, Gonçalves, Roberto, Fernandez-Lafuente

Publikováno v: Frontiers in Bioengineering and Biotechnology

The lipase from Pseudomonas fluorescens (PFL) has been immobilized on octyl-agarose beads under 16 different conditions (varying pH, ionic strength, buffer, adding some additives) at two different loadings, 1 and 60 mg of enzyme/g of support with the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d8df08f191e64ba78a5765c95476535d
https://pubmed.ncbi.nlm.nih.gov/32181245

Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production

Autor: Sara Arana-Peña, Roberto Fernandez-Lafuente, Yuliya Lokha, Nathalia Saraiva Rios, Luciana Rocha Barros Gonçalves, Carmen Mendez-Sanchez

Publikováno v: Repositório Institucional da UFRN
Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN

2030-12 A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8eddd91956748cc87c0a863442774bb
https://doi.org/10.1016/j.enzmictec.2020.109535

Immobilization on octyl-agarose beads and some catalytic features of commercial preparations of lipase a from Candida antarctica (Novocor ADL): Comparison with immobilized lipase B from Candida antarctica

Autor: Roberto Fernandez-Lafuente, Sara Arana-Peña, Yuliya Lokha

Publikováno v: Biotechnology progress. 35(1)

Lipase A from Candida antarctica (CALA, commercialized as Novocor ADL) was immobilized on octyl-agarose, which is a very useful support for lipase immobilization, and coated with polyethylenimine to improve the stability. The performance was compared

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b1e5a6d7e55a2a6f1c08f58750a4cc5
https://pubmed.ncbi.nlm.nih.gov/30341806