Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Yukimasa Makita"'
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-9 (2024)
Abstract NGLY1 deficiency is a genetic disease caused by biallelic mutations of the Ngly1 gene. Although epileptic seizure is one of the most severe symptoms in patients with NGLY1 deficiency, preclinical studies have not been conducted due to the la
Externí odkaz:
https://doaj.org/article/af6ac118a0734d2081d884d851b53a76
Autor:
Eriya Kenjo, Hiroyuki Hozumi, Yukimasa Makita, Kumiko A. Iwabuchi, Naoko Fujimoto, Satoru Matsumoto, Maya Kimura, Yuichiro Amano, Masataka Ifuku, Youichi Naoe, Naoto Inukai, Akitsu Hotta
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
In vivo delivery of CRISPR-Cas9 holds promise for treating muscular dystrophy, however, AAV delivery is known to be immunogenic. Here, the authors show that LNP delivery of CRISPR-Cas9 enables repeated injections into skeletal muscle and leads to res
Externí odkaz:
https://doaj.org/article/82ba1424688643b8923145dd7be8e610
Autor:
Peter Gee, Mandy S. Y. Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F. Yang, Mio Iwasaki, Xiou H. Wang, Matthew A. Waller, Nanako Shirai, Yasuko O. Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita, Kumiko A. Iwabuchi, Masahiko Yasuda, Huaigeng Xu, Takeshi Noda, Jun Komano, Hidetoshi Sakurai, Naoto Inukai, Akitsu Hotta
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Expression of Cas9 and gRNA from viral vectors in vivo may cause off-target activity. Here the authors present NanoMEDIC, which uses nanovesicles to transiently deliver editing machinery to hard-to-transfect cells.
Externí odkaz:
https://doaj.org/article/4d0fe2ef9a604f0fb6865d1e08b1ed50
Autor:
Kumiko Iwabuchi, Akihiro Kagita, Lucy F. Yang, Nanako Shirai, Takahiro Iguchi, Yoko Fujita, Jun Komano, Takeshi Noda, Akitsu Hotta, Noriko Sasakawa, Yukimasa Makita, Matthew A. Waller, Naoto Inukai, Peter Gee, Mio Iwasaki, Mandy S. Y. Lung, Hidetoshi Sakurai, Yasuko O. Abe, Xiou H. Wang, Yasutomo Miura, Kei Watanabe, Masahiko Yasuda, Hiroyuki Hozumi, Huaigeng Xu, Yuya Okuzaki
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Nature Communications
Nature Communications
Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is needed for therapeutic genome editing applications. Here, we develop an extracellul
Autor:
Hiroshi Uejima, Yoshiki Katou, Yuji Kawamata, Shumpei Murata, Kuniko Kikuchi, Satoru Matsumoto, Kentaro Otake, Mika Teratani, Yasutaka Hoashi, Kenjo Eriya, Masahiro Nogami, Yukimasa Makita
Publikováno v:
Biochemical and Biophysical Research Communications. 493:800-806
Hepatocellular carcinoma (HCC) is still one of the major causes of cancer-related death. Kinetochore-associated protein 2 (KNTC2) is specifically upregulated in tumor tissues of HCC patients and recognized as a potential candidate target for the trea
Autor:
Mika Teratani, Shuichi Takagahara, Takayuki Kamei, Yasushi Masuda, Sachiko Imaichi, Satoshi Nishimura, Shota Ikeda, Shumpei Murata, Tetsuya Ohtaki, Tatsuo Oikawa, Kenjo Eriya, Kenichi Miyata, Yukimasa Makita, Kentaro Otake, Tsukasa Sugo, Kuniko Kikuchi, Mari Ogasawara-Shimizu, Hirokazu Matsumoto, Michiko Terada
Publikováno v:
Journal of Controlled Release. 237:1-13
Despite considerable efforts to develop efficient carriers, the major target organ of short-interfering RNAs (siRNAs) remains limited to the liver. Expanding the application outside the liver is required to increase the value of siRNAs. Here we repor
Autor:
Shumpei Murata, Yuji Kawamata, Mika Teratani, Satoru Matsumoto, Yukimasa Makita, Yasutaka Hoashi
Publikováno v:
Oncology letters. 15(4)
It has been widely reported that patient-derived tumor xenografts (PDXs) are more similar to tumor tissues than conventional cancer cell lines. Kinetochore-associated protein 2 (KNTC2) is known to be upregulated specifically in tumor tissues of cance
Publikováno v:
Biochemical and biophysical research communications. 486(3)
Endoplasmic reticulum (ER) stress caused by accumulation of misfolded proteins is observed in several kinds of diseases. Since ER stress is reported to be involved in the progression of non-alcoholic steatohepatitis (NASH), highly sensitive and simpl
Publikováno v:
Clinical calcium. 27(3)
Recent advances in genome editing technologies have opened the possibility for treating genetic diseases, such as Duchenne muscular dystrophy(DMD), by correcting the causing gene mutations in dystrophin gene. In fact, there are several reports that d
Publikováno v:
Journal of bioscience and bioengineering. 92(3)
Green fluorescent protein (GFP) reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourt