Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Yuki Kiguchi"'
Autor:
Yuki Kiguchi, Hiroyuki Oyama, Izumi Morita, Yasuhiro Nagata, Naoko Umezawa, Norihiro Kobayashi
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Abstract In vitro affinity-maturation potentially generates antibody fragments with enhanced antigen-binding affinities that allow for developing more sensitive diagnostic systems and more effective therapeutic agents. Site-directed mutagenesis targe
Externí odkaz:
https://doaj.org/article/8bb6256cd6294d32b16c979a6b21bdcb
Autor:
Yuki, Kiguchi
Publikováno v:
YAKUGAKU ZASSHI. 142:1153-1159
Antibody engineering is a powerful method used to generate high-affinity antibodies that enables sensitive immunoassays. It is commonly performed with the following steps: First, antibody fragments (e.g., single-chain Fv fragments; scFvs) with variou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae6954f821d247096b43a86c617ce82f
https://doi.org/10.1248/yakushi.22-00141
https://doi.org/10.1248/yakushi.22-00141
Autor:
Izumi Morita, Yuki Kiguchi, Saya Nakamura, Ayano Yoshida, Haruna Kubo, Momo Ishida, Hiroyuki Oyama, Norihiro Kobayashi
Publikováno v:
Biological and Pharmaceutical Bulletin. 45:851-855
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::820b96623284710cfff5aa6344add663
https://doi.org/10.1248/bpb.b22-00187
https://doi.org/10.1248/bpb.b22-00187
Publikováno v:
Biologicalpharmaceutical bulletin. 45(10)
Previously, we generated high-affinity antibody mutants that enabled sensitive immunoassays by exploring diverse libraries of single-chain Fv fragments (scFvs) displayed on bacteriophage. To isolate rarely-occurring desirable clones, "panning" has co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b874e865badcaa85e485e4b1ecf28d8b
https://pubmed.ncbi.nlm.nih.gov/36184500
https://pubmed.ncbi.nlm.nih.gov/36184500
Autor:
Kouya Yamaki, Kiyoe Ohta, Norihiro Kobayashi, Izumi Morita, Yuki Kiguchi, Hiroyuki Oyama, Ken Ito, Asuka Nanbo, Hirozo Oh-oka, Yutaka Koyama, Yoshiki Kawata, Hirotaka Fujisawa, Mitsuhiro Ohta
Publikováno v:
Biologicalpharmaceutical bulletin. 45(8)
The emu is the second largest ratite; thus, their sera and egg yolks, obtained after immunization, could provide therapeutic and diagnostically important immunoglobulins with improved production efficiency. Reliable purification tools are required to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b3e23ffa2e0f4ed9ba6d7316d9f5267
https://pubmed.ncbi.nlm.nih.gov/35908885
https://pubmed.ncbi.nlm.nih.gov/35908885
Autor:
Izumi Morita, Yuki Kiguchi, Hiroyuki Oyama, Kouya Yamaki, Nami Sakio, Keisuke Kashiwabara, Yumi Kuroda, Aya Ito, Asaka Yokota, Natsumi Ikeda, Ruri Kikura-Hanajiri, Hiroshi Ueda, Satoshi Numazawa, Takemi Yoshida, Norihiro Kobayashi
Publikováno v:
Analytical methods : advancing methods and applications. 14(28)
Chemical derivatization into larger molecules has generated a monoclonal antibody that enables group-specific immunochemical detection of potent methamphetamine and amphetamine enantiomers.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55de73a0289c894c94c2279e20092ff8
https://pubmed.ncbi.nlm.nih.gov/35785801
https://pubmed.ncbi.nlm.nih.gov/35785801
Autor:
Yuki Kiguchi, Hiroyuki Oyama, Chisato Tode, Norihiro Kobayashi, Izumi Morita, Ruri Kikura-Hanajiri, Atsuko Takeuchi, Jun Ogata, Rie Tanaka
Publikováno v:
Analytical Methods. 13:3954-3962
A sensitive immunochemical method for identifying hallucinogenic mushrooms (magic mushrooms) is required for regulating their illicit use. We have previously generated a monoclonal antibody (mAb) that targets psilocin (Psi), the major psychoactive co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::044ecdb34bea1adb61e25533dba81cf3
https://doi.org/10.1039/d1ay01157j
https://doi.org/10.1039/d1ay01157j
Autor:
Yasuhiro Nagata, Norihiro Kobayashi, Izumi Morita, Naoko Umezawa, Yuki Kiguchi, Hiroyuki Oyama
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Scientific Reports
Scientific Reports
In vitro affinity-maturation potentially generates antibody fragments with enhanced antigen-binding affinities that allow for developing more sensitive diagnostic systems and more effective therapeutic agents. Site-directed mutagenesis targeting “h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffb5367c298abe1f09b34393cde080ca
https://doi.org/10.1038/s41598-021-87501-7
https://doi.org/10.1038/s41598-021-87501-7
Autor:
Norihiro Kobayashi, Yuki Kiguchi, Yuka Higashi, Atsuko Takeuchi, Yuri Enami, Miku Taguchi, Hanako Hasegawa, Tatsuya Tagawa, Chika Yamamoto, Hiroyuki Oyama, Yuriko Takamine, Izumi Morita
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Scientific Reports
Scientific Reports
“Antibody-breeding” has provided therapeutic/diagnostic antibody mutants with greater performance than native antibodies. Typically, random point mutations are introduced into the VH and VL domains of parent antibodies to generate diverse librari
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c901fedf60f3ef09395800beeea008b5
https://doi.org/10.1038/s41598-020-61529-7
https://doi.org/10.1038/s41598-020-61529-7
Autor:
Mai Narasaki, Yuki Kiguchi, Emiko Katayama, Norihiro Kobayashi, Aiko Yokoyama, Masatoshi Fujita, Izumi Morita, Hiroyuki Oyama
Publikováno v:
Biological and Pharmaceutical Bulletin. 41:1062-1070
Antibodies are essential for characterizing various analytes. "Molecular-breeding" approaches enable rapid generation of antibody mutants with desirable antigen-binding abilities. Typically, prototype antibodies are converted to single-chain Fv fragm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77daca5e1432bace1043240d39ab6251
https://doi.org/10.1248/bpb.b18-00162
https://doi.org/10.1248/bpb.b18-00162