Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Yuiko Sahara-Miura"'
Autor:
Satoshi Inouye, Jun-ichi Sato, Yuiko Sahara-Miura, Yuri Tomabechi, Yuto Sumida, Shun-ichi Sekine, Mikako Shirouzu, Takamitsu Hosoya
Publikováno v:
PLoS ONE, Vol 17, Iss 9 (2022)
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs. Mutated wild KAZ with 16 amino acid substitutions (nanoKAZ/nanoLuc) utilizes bis-coelent
Externí odkaz:
https://doaj.org/article/0ab9804ceac14a5fa5afb2bd5e36d483
Autor:
Satoshi Inouye, Yuiko Sahara-Miura
Publikováno v:
Protein Expression and Purification. 137:58-63
Aequorin is a Ca 2+ -binding photoprotein that is a complex of apoaequorin (apoAQ) and 2-peroxycoelenterazine. In this study, the fusion protein (ZZ-apoAQ) composed of the synthetic IgG-binding domain (ZZ domain) derived from Staphylococcus aureus pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6570c8f20b129a0d47c85bfb4cd05e0
https://doi.org/10.1016/j.pep.2017.06.017
https://doi.org/10.1016/j.pep.2017.06.017
Autor:
Satoshi Inouye1 sinouye@jnc-corp.co.jp, Yuiko Sahara-Miura1
Publikováno v:
Journal of Biochemistry. Jul2016, Vol. 160 Issue 1, p59-68. 10p.
Autor:
Yuiko Sahara-Miura, Satoshi Inouye
Publikováno v:
Journal of Biochemistry. 160:59-68
The binding properties of Ca(2+) to EF-hand I of aequorin (AQ) were characterized by replacing the loop sequence of EF-hand I (AQ[I]) with other known loop sequences of Ca(2+)-binding proteins, including photoproteins (aequorin, clytin-I, clytin-II a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::325d91d0d9950dc4684abdc7275ee373
https://doi.org/10.1093/jb/mvw013
https://doi.org/10.1093/jb/mvw013
Publikováno v:
Biochemical and Biophysical Research Communications. 450:1313-1319
The putative amino-terminal signal peptide of the catalytic 19 kDa protein (KAZ) of Oplophorus luciferase was found to be a functional secretory peptide in mammalian cells. A 16 amino acid substituted mutant of KAZ (nanoKAZ) could be secreted from ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6cc03d2443fb1e4ca992e229fabfcc9
https://doi.org/10.1016/j.bbrc.2014.06.140
https://doi.org/10.1016/j.bbrc.2014.06.140
Publikováno v:
Biochemical and Biophysical Research Communications. 445:157-162
To characterize the luminescence properties of nanoKAZ, a 16 amino acid substituted mutant of the catalytic 19 kDa protein (KAZ) of Oplophorus luciferase, the effects of each mutated amino acid were investigated by site-specific mutagenesis. All 16 s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4656738959dc0cc7a6722a26ed9b126
https://doi.org/10.1016/j.bbrc.2014.01.133
https://doi.org/10.1016/j.bbrc.2014.01.133
Publikováno v:
Biochemical and Biophysical Research Communications. 410:792-797
The mutated recombinant Gaussia luciferase (hgGLase) having the hinge sequence with a reactive cysteine residue at the carboxyl terminal region was purified from Escherichia coli cells by nickel-chelate affinity chromatography and hydrophobic chromat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86bfe983649adae5baefde07a9cab17c
https://doi.org/10.1016/j.bbrc.2011.06.063
https://doi.org/10.1016/j.bbrc.2011.06.063
Autor:
Yuiko Sahara-Miura, Satoshi Inouye
Publikováno v:
Biochemical and biophysical research communications. 469(2)
Clytin-II (CL-II) is an isotype of the calcium-binding photoprotein clytin-I (CL-I) from Clytia gregaria. CL-II shows approximately 4.5-fold higher initial luminescence intensity than CL-I with the same luminescence capacity, and is a potential candi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85aa53ac316a9b61b04f667b37802a25
https://pubmed.ncbi.nlm.nih.gov/26626073
https://pubmed.ncbi.nlm.nih.gov/26626073
Autor:
Satoshi Inouye, Suguru Yoshida, Yuto Sumida, Jun Ichi Sato, Yuiko Sahara-Miura, Takamitsu Hosoya, Rie Iimori
Publikováno v:
Organic letters. 17(15)
A novel synthetic method for v-coelenterazine (v-CTZ), which is a vinylene-bridged analog of native CTZ with a large red-shifted luminescence property, is described. The synthesis was achieved in a concise way through the use of three sequential cros
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::236d37a14e9446ad84dbb2f65f3e32ff
https://pubmed.ncbi.nlm.nih.gov/26194904
https://pubmed.ncbi.nlm.nih.gov/26194904
Publikováno v:
Protein expression and purification. 109
A simple design method for codon optimization of genes to express a heterologous protein in mammalian cells is described. Codon optimization was performed by choosing only codons preferentially used in humans and with over 60% GC content, and the met
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0585299aae6cf390717e209161e0ced
https://pubmed.ncbi.nlm.nih.gov/25665506
https://pubmed.ncbi.nlm.nih.gov/25665506