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New Platform for Screening Genetic Libraries at Elevated Temperatures: Biological and Genomic Information and Genetic Tools of Geobacillus thermodenitrificans K1041

Autor: Kosuke Koyama, Yui Mikawa, Shota Nakagawa, Ryota Kurashiki, Takashi Ohshiro, Hirokazu Suzuki

Publikováno v: Appl Environ Microbiol

Geobacillus thermodenitrificans K1041 is an unusual thermophile that is highly transformable via electroporation, making it a promising host for screening genetic libraries at elevated temperatures. In this study, we determined its biological propert

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37c6c195d7b1fd8b24d20fadadf2aa24
https://europepmc.org/articles/PMC9499010/

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Transcriptome and growth efficiency comparisons of recombinant thermophiles that produce thermolabile and thermostable proteins: implications for burden-based selection of thermostable proteins

Autor: Shunsuke Yoshimura, Hirokazu Suzuki, Mao Takata, Takashi Ohshiro, Yui Mikawa, Yuta Okumura

Publikováno v: Extremophiles : life under extreme conditions. 25(4)

Geobacillus kaustophilus is a thermophilic bacterium that grows at temperatures ranging between 42 and 74 °C. Here, we modified this organism to produce the thermolabile protein (PyrFA) or its thermostable variant (PyrFV) and analyzed the transcript

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b638d6d8dd7539dc1638cec1d0e7228
https://pubmed.ncbi.nlm.nih.gov/34191121

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