Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Yui, Tomioka"'
Autor:
Tsutomu Arakawa, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, Teruo Akuta
Publikováno v:
Polysaccharides, Vol 5, Iss 3, Pp 380-398 (2024)
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecu
Externí odkaz:
https://doaj.org/article/d7c938d31bd649848d40d0aecd02fd23
Autor:
Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta
Publikováno v:
BioTechniques, Vol 72, Iss 5, Pp 207-218 (2022)
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provid
Externí odkaz:
https://doaj.org/article/617c78fb8b3e44e2b265eac57674e82c
Autor:
Tsutomu Arakawa, Yui Tomioka, Masataka Nakagawa, Chiaki Sakuma, Yasunori Kurosawa, Daisuke Ejima, Kouhei Tsumoto, Teruo Akuta
Publikováno v:
Antibodies, Vol 12, Iss 1, p 15 (2023)
Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are
Externí odkaz:
https://doaj.org/article/cac5b1d7ce30488dba3045111abd0851
Autor:
Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa
Publikováno v:
Antibodies, Vol 11, Iss 2, p 36 (2022)
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the
Externí odkaz:
https://doaj.org/article/906228a9cdd34f64873d8605805429af
Publikováno v:
International Journal of Biological Macromolecules. 198:26-36
Solvent additives, including NaCl, arginine hydrochloride (ArgHCl), glycine and sucrose, are used to enhance protein stability or reduce protein aggregation. Here, we studied the effects of these additives on proteins using agarose native gel electro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b586972e79880738d2705067ba9943b9
https://doi.org/10.1016/j.ijbiomac.2021.12.084
https://doi.org/10.1016/j.ijbiomac.2021.12.084
Autor:
Teruo Akuta, Takashi Shibata, Masataka Nakagawa, Chiaki Sakuma, Yasuo Ono, Yasunori Kurosawa, Yoshinori Sato, Ryo Sato, Yui Tomioka, Tsutomu Arakawa
Publikováno v:
International Journal of Biological Macromolecules. 189:869-878
Electrophoresis is one of the major techniques to analyze macromolecular structure and interaction. Its capability depends on the sensitivity and specificity of the staining methods. We have here examined silver staining of proteins and nucleic acids
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85ac8c31d8de0e7a4e30319e097690c1
https://doi.org/10.1016/j.ijbiomac.2021.08.142
https://doi.org/10.1016/j.ijbiomac.2021.08.142
Publikováno v:
Journal of Chromatography A. :464091
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf3679e790b60075d5694184bee859d1
https://doi.org/10.1016/j.chroma.2023.464091
https://doi.org/10.1016/j.chroma.2023.464091
Autor:
Yui Tomioka, Ryo Sato, Ryo Takahashi, Satoru Nagatoishi, Kohei Shiba, Kouhei Tsumoto, Tsutomu Arakawa, Teruo Akuta
Publikováno v:
Biophysical Chemistry. 296:106977
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ca34831d547d622d1f66cd213bfccd05
https://doi.org/10.1016/j.bpc.2023.106977
https://doi.org/10.1016/j.bpc.2023.106977
Autor:
Tsutomu Arakawa, Yasunori Kurosawa, Chiaki Sakuma, Cynthia Li, Takashi Shibata, Teruo Akuta, Masataka Nakagawa, Yui Tomioka
Publikováno v:
International Journal of Biological Macromolecules. 172:589-596
Agarose native gel electrophoresis has been developed to separate proteins and protein complexes in the native state. Here, we applied this technology to analyze proteins that undergo degradation, post-translational modification or chemical/physical
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d8b400d18d79bb069035dee26c9ec05
https://doi.org/10.1016/j.ijbiomac.2021.01.075
https://doi.org/10.1016/j.ijbiomac.2021.01.075