Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Yuan Chang Yan"'
Publikováno v:
Cell Research. 17:163-173
Cyclin-dependent kinase 7 (Cdk7) is the catalytic subunit of the metazoan Cdk-activating kinase (CAK). Activation of Cdk7 requires its association with a regulatory subunit, Cyclin H. Although the Cdk7/Cyclin H complex has been implicated in the regu
Autor:
Wu-Wen, Zhang, Yun-Bin, Zhang, Xin-Xi, Zhao, Ye, Hua, Zhi-Li, Wu, Yuan-Chang, Yan, Yi-Ping, Li
Publikováno v:
Journal of molecular cell biology. 7(5)
Publikováno v:
Journal of Virological Methods. 134:48-54
The strategy that transcribes short hairpin RNAs (shRNAs) by RNA polymerase II promoters is expected to present flexible approaches for regulating the patterns of shRNA expression. The capacity of generating shRNA by a modified adenovirus RNA polymer
Publikováno v:
Neurochemical Research. 27:195-199
GABA triggers mammalian sperm acrosome reaction (AR). Here, evidence is presented, showing that rat spermatozoa contain GABAA receptors, composed of α5, β1 and β3 subunits. The effects of GABAA receptor agonist and antagonist on the induction of A
Autor:
Yuan Chang Yan, Mary M.Y. Waye, Stephen Kwok-Wing Tsui, W.L Wu, Kwok-Pui Fung, S.S Siu, S.C. So, Y.W. Chung, Xu Wang, H.C. Chan, S.C.W Luk, Cheuk Yu Lee, L.L Tsang
Publikováno v:
Biochemical and Biophysical Research Communications. 270:581-587
We have previously reported an association of 14-3-3epsilon isoform with calmodulin. Using the voltage-clamp technique, the present study investigated the potential role of 14-3-3 in modulating the Ca(2+)-activated Cl(-) channel (CaCC) endogenously e
Publikováno v:
Cell Research. 10:51-58
Some recent studies indicated that GABAergic system is involved in mammalian sperm acrosome reaction (AR), but direct evidence pertaining to the expression of gat1 in mammalian sperm is not yet demonstrated. In this study, we evaluated the presence o
Publikováno v:
Cell Research. 7:51-59
Epidermal growth factor (EGF) is produced primarily by Leydig cells of human testis. Expression of the EGF gene was assessed in mouse testis during the course of sexual maturation by the application of the RT-PCR method and the use of specific oligon
Publikováno v:
Molecular Biology Reports. 21:105-112
A secretory high-level expression cloning vector designated as pSBC-20 was constructed by inserting a DNA fragment encoding the signal peptide of ompA protein into pBV 220 vector. Any foreign DNA fragment can be inserted into the polylinker cloning s
Publikováno v:
Archives of Andrology. 28:91-97
The cDNA (RSD-2) encoding a human sperm protein (YWK-II) was isolated from a rat testis lambda gt11 expression library. Its nucleotide sequence was determined. The deduced polypeptide showed high identity with the transmembrane-cytoplasmic domains of
Publikováno v:
Proceedings of the National Academy of Sciences. 87:2405-2408
A rat testis lambda gt11 cDNA library was screened with a monoclonal antibody raised against a human sperm membrane protein designated YWK-II. A clone was found with a cDNA insert composed of 1837 base pairs that contained an open reading frame codin