Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Yu. G. Kashirina"'
Publikováno v:
Molecular Biology. 43:8-15
The operon for the Bacillus stearothermophilus SE-589 nickase-modification system (NM.BstSEI, recognition site 5′-GAGTC-3′) includes two DNA methyltransferase (M.) genes, bstSEIM1 and bstSEIM2. The gene encoding M2.BstSEI was cloned in pJW and ex
Autor:
E. V. Chmuzh, Yu. G. Kashirina, Yu. E. Tomilova, V. A. Chernukhin, S. S. Okhapkina, D. A. Gonchar, V. S. Dedkov, M. A. Abdurashitov, S. Kh. Degtyarev
Publikováno v:
Molecular Biology. 41:37-43
Genes coding for the Flavobacterium sp. 4H restriction-modification (RM) system, which recognizes the sequence 5′-GCNGC-3′, were cloned in Escherichia coli ER2267 and sequenced. The Fsp4HI RM system includes two genes: one for DNA methyltransfera
Autor:
S. Kh. Degtyarev, D. A. Gonchar, L. N. Golikova, N. A. Netesova, Abdurashitov Ma, Yu. G. Kashirina, Valery A Chernukhin
Publikováno v:
Biochemistry (Moscow). 68:967-975
The BstF5I restriction–modification system from Bacillus stearothermophilus F5 includes four site-specific DNA methyltransferases, thus differing from all known restriction–modification systems. Here we demonstrated for the first time that one ba
Autor:
N. A. Netesova, V. V. Kuznetsov, S. Kh. Degtyarev, D. A. Gonchar, Yu. G. Kashirina, Valery A Chernukhin
Publikováno v:
Biochemistry. Biokhimiia. 75(1)
Optimal conditions for DNA methylation by the M3.BstF5I enzyme from Bacillus stearothermophilus and kinetic parameters of lambda phage DNA modification and that of a number of oligonucleotide substrates are established. Comparison of M1.BstF5I and M3
Autor:
Valery A Chernukhin, Yu. G. Kashirina, K. S. Sukhanova, D. A. Gonchar, M. A. Abdurashitov, S. Kh. Degtyarev
Publikováno v:
Scopus-Elsevier
A gene encoding DNA methyltransferase (methylase) FauIA of the restriction-modification system FauI from Flavobacterium aquatile (recognizing sequence 5'-CCCGC-3') was cloned in pJW vector. The latter was used for transformation of E. coli RRI cells
Autor:
V A, Chernukhin, L N, Golikova, D A, Gonchar, M A, Abdurashitov, Yu G, Kashirina, N A, Netesova, S Kh, Degtyarev
Publikováno v:
Biochemistry. Biokhimiia. 68(9)
The BstF5I restriction-modification system from Bacillus stearothermophilus F5 includes four site-specific DNA methyltransferases, thus differing from all known restriction-modification systems. Here we demonstrated for the first time that one bacter