Zobrazeno 1 - 10
of 64
pro vyhledávání: '"Yu Ishikawa"'
Multiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice
Autor:
Tsutomu Endo, Kiyonori Kobayashi, Takafumi Matsumura, Chihiro Emori, Manabu Ozawa, Shimpei Kawamoto, Daisuke Okuzaki, Keisuke Shimada, Haruhiko Miyata, Kentaro Shimada, Mayo Kodani, Yu Ishikawa-Yamauchi, Daisuke Motooka, Eiji Hara, Masahito Ikawa
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-16 (2024)
Abstract In mammals, females undergo reproductive cessation with age, whereas male fertility gradually declines but persists almost throughout life. However, the detailed effects of ageing on germ cells during and after spermatogenesis, in the testis
Externí odkaz:
https://doaj.org/article/623ca58762e34a14a21188196ebbee61
Autor:
Takehiko OGAWA, Takafumi MATSUMURA, Tatsuma YAO, Hiroshi KIMURA, Kiyoshi HASHIMOTO, Yu ISHIKAWA-YAMAUCHI, Takuya SATO
Publikováno v:
The Journal of Reproduction and Development, Vol 70, Iss 1, Pp 1-9 (2023)
Incorporation of bovine serum-derived albumin formulation (AlbuMAX) into a basic culture medium, MEMα, enables the completion of in vitro spermatogenesis through testicular tissue culture in mice. However, this medium was not effective in other anim
Externí odkaz:
https://doaj.org/article/3934235b85474ecd9faf744d9bf2c0a3
Autor:
Kiyoshi Hashimoto, Hisakazu Odaka, Yu Ishikawa-Yamauchi, Shino Nagata, Hiroko Nakamura, Hiroshi Kimura, Takuya Sato, Kazuhide Makiyama, Takehiko Ogawa
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)
Abstract The classical organ culture method, in which tissue is placed at the gas‒liquid interphase, is effective at inducing mouse spermatogenesis. However, due to reginal variations in the supply of oxygen and nutrients within a tissue, the progr
Externí odkaz:
https://doaj.org/article/c00ed94f705a4ea883a1835418e61435
Autor:
Takafumi Matsumura, Kumiko Katagiri, Tatsuma Yao, Yu Ishikawa-Yamauchi, Shino Nagata, Kiyoshi Hashimoto, Takuya Sato, Hiroshi Kimura, Takashi Shinohara, Makoto Sanbo, Masumi Hirabayashi, Takehiko Ogawa
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)
Abstract An in vitro spermatogenesis method using mouse testicular tissue to produce fertile sperm was established more than a decade ago. Although this culture method has generally not been effective in other animal species, we recently succeeded in
Externí odkaz:
https://doaj.org/article/840c25e053eb49ef8f12a549efe24686
Autor:
Anh Hoang Pham, Chihiro Emori, Yu Ishikawa-Yamauchi, Keizo Tokuhiro, Maki Kamoshita, Yoshitaka Fujihara, Masahito Ikawa
Publikováno v:
Cells, Vol 13, Iss 10, p 802 (2024)
Infertility is considered a global health issue as it currently affects one in every six couples, with female factors reckoned to contribute to partly or solely 50% of all infertility cases. Over a thousand genes are predicted to be highly expressed
Externí odkaz:
https://doaj.org/article/10fc31a375564485a4e494ebab4414a2
Autor:
Manabu Ozawa, Hideto Mori, Tsutomu Endo, Yu Ishikawa-Yamauchi, Daisuke Motooka, Chihiro Emori, Masahiro Ikawa
Publikováno v:
iScience, Vol 26, Iss 12, Pp 108456- (2023)
Summary: Male fertility decreases with aging, with spermatogenic decline being one of its causes. Altered testis environment is suggested as a cause of the phenotype; however, the associated mechanisms remain unclear. Herein, we investigated the age-
Externí odkaz:
https://doaj.org/article/fb2a7fd3eb684b9eb58f659567a5a555
Autor:
Manabu Ozawa, Jumpei Taguchi, Kento Katsuma, Yu Ishikawa-Yamauchi, Mio Kikuchi, Reiko Sakamoto, Yasuhiro Yamada, Masahito Ikawa
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)
Abstract Gene targeting of embryonic stem (ES) cells followed by chimera production has been conventionally used for developing gene-manipulated mice. Although direct knock-in (KI) using murine zygote via CRISPR/Cas9-mediated genome editing has been
Externí odkaz:
https://doaj.org/article/e53033ea683742beaa843bd39da7ec56
Autor:
Manami SENOO, Hiroshi HOZOJI, Yu ISHIKAWA-YAMAUCHI, Takashi TAKIJIRI, Sho OHTA, Tomoyo UKAI, Mio KABATA, Takuya YAMAMOTO, Yasuhiro YAMADA, Masahito IKAWA, Manabu OZAWA
Publikováno v:
The Journal of Reproduction and Development, Vol 66, Iss 5, Pp 459-467 (2020)
PTBP1, a well-conserved RNA-binding protein, regulates cellular development by tuning posttranscriptional mRNA modification such as alternative splicing (AS) or mRNA stabilization. We previously revealed that the loss of Ptbp1 in spermatogonia causes
Externí odkaz:
https://doaj.org/article/b663433465594b679f115f4d9a3ebd35
Autor:
Hossam H Shawki, Yu Ishikawa-Yamauchi, Akihiro Kawashima, Yuki Katoh, Manabu Matsuda, Al-Sayed Al-Soudy, Fatma M Minisy, Akihiro Kuno, Xiafukaiti Gulibaikelamu, Takatsugu Hirokawa, Satoru Takahashi, Hisashi Oishi
Publikováno v:
PLoS ONE, Vol 14, Iss 4, p e0214687 (2019)
Calcium-binding proteins regulate ion metabolism and the necessary signaling pathways for the maturational events of sperm. Our aim is to identify the novel calcium-binding proteins in testis. The gene EFCAB2 (GenBank NM_026626.3, NP_080902.1) was no
Externí odkaz:
https://doaj.org/article/8775523a41db42789fc4d1c2bd2c3de4
Autor:
Takehiko OGAWA, Takafumi MATSUMURA, Tatsuma YAO, Hiroshi KIMURA, Kiyoshi HASHIMOTO, Yu ISHIKAWA-YAMAUCHI, Takuya SATO
Publikováno v:
Journal of Reproduction & Development; 2024, Vol. 70 Issue 1, p1-9, 9p