OptoREACT: Optogenetic Receptor Activation on Nonengineered Human T Cells.

Autor: Armbruster A; INM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken 66123, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany., Ehret AK; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Albertstr. 19A, Freiburg 79104, Germany., Russ M; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany., Idstein V; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Albertstr. 19A, Freiburg 79104, Germany., Klenzendorf M; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany., Gaspar D; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany., Juraske C; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Albertstr. 19A, Freiburg 79104, Germany., Yousefi OS; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany., Schamel WW; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, and Faculty of Medicine, University of Freiburg, Breisacher Str. 115, Freiburg 79106, Germany., Weber W; INM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken 66123, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.; Department of Materials Science and Engineering, Saarland University, Campus D2 2, Saarbrücken 66123, Germany., Hörner M; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestr. 18, Freiburg 79104, Germany.; Faculty of Biology, University of Freiburg, Schänzlestr. 1, Freiburg 79104, Germany.

Publikováno v: ACS synthetic biology [ACS Synth Biol] 2024 Mar 15; Vol. 13 (3), pp. 752-762. Date of Electronic Publication: 2024 Feb 09.

Opto-APC: Engineering of cells that display phytochrome B on their surface for optogenetic studies of cell-cell interactions.

Autor: Russ M; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany., Ehret AK; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany., Hörner M; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany., Peschkov D; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany., Bohnert R; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany., Idstein V; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany., Minguet S; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany., Weber W; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany., Lillemeier BF; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany., Yousefi OS; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany., Schamel WW; Signalling Research Centres BIOSS and CIBSS, Faculty of Biology, University of Freiburg, Freiburg, Germany.; Centre for Chronic Immunodeficiency (CCI), Medical Centre Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Publikováno v: Frontiers in molecular biosciences [Front Mol Biosci] 2023 Feb 20; Vol. 10, pp. 1143274. Date of Electronic Publication: 2023 Feb 20 (Print Publication: 2023).

Cross-TCR Antagonism Revealed by Optogenetically Tuning the Half-Life of the TCR Ligand Binding.

Autor: Yousefi OS; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, 79110 Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University Freiburg, 79104 Freiburg, Germany., Ruggieri M; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, 79110 Freiburg, Germany.; Department of Pathology, Faculty of Medicine, University of Freiburg, 79110 Freiburg, Germany., Idstein V; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, 79110 Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University Freiburg, 79104 Freiburg, Germany., von Prillwitz KU; Institute of Physics, University of Freiburg, 79104 Freiburg, Germany.; Freiburg Center for Data Analysis and Modeling (FDM), University of Freiburg, 79104 Freiburg, Germany., Herr LA; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, 79110 Freiburg, Germany., Chalupsky J; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, 79110 Freiburg, Germany., Köhn M; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany., Weber W; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University Freiburg, 79104 Freiburg, Germany., Timmer J; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany.; Institute of Physics, University of Freiburg, 79104 Freiburg, Germany.; Freiburg Center for Data Analysis and Modeling (FDM), University of Freiburg, 79104 Freiburg, Germany., Schamel WWA; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, 79104 Freiburg, Germany.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, 79110 Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University Freiburg, 79104 Freiburg, Germany.

Publikováno v: International journal of molecular sciences [Int J Mol Sci] 2021 May 06; Vol. 22 (9). Date of Electronic Publication: 2021 May 06.

Noncanonical binding of Lck to CD3ε promotes TCR signaling and CAR function.

Autor: Hartl FA; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, Freiburg, Germany., Beck-Garcìa E; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany., Woessner NM; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University Freiburg, Freiburg, Germany., Flachsmann LJ; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany., Cárdenas RMV; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Brandl SM; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University Freiburg, Freiburg, Germany., Taromi S; Department of Medicine I, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.; Faculty of Medical and Life Sciences, Furtwangen University, Campus VS-Schwenningen, Furtwangen, Germany., Fiala GJ; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Morath A; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University Freiburg, Freiburg, Germany., Mishra P; Pharmaceutical Bioinformatics, Albert-Ludwigs-University of Freiburg, Freiburg, Germany., Yousefi OS; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University Freiburg, Freiburg, Germany., Zimmermann J; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany., Hoefflin N; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Köhn M; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Wöhrl BM; Biopolymers, University of Bayreuth, Bayreuth, Germany., Zeiser R; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.; Department of Medicine I, Medical Center, University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany., Schweimer K; Biopolymers, University of Bayreuth, Bayreuth, Germany., Günther S; Pharmaceutical Bioinformatics, Albert-Ludwigs-University of Freiburg, Freiburg, Germany., Schamel WW; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, Freiburg, Germany., Minguet S; Faculty of Biology, Albert-Ludwigs-University of Freiburg, Freiburg, Germany. susana.minguet@biologie.uni-freiburg.de.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany. susana.minguet@biologie.uni-freiburg.de.; Center of Chronic Immunodeficiency CCI, University Clinics and Medical Faculty, Freiburg, Germany. susana.minguet@biologie.uni-freiburg.de.

Publikováno v: Nature immunology [Nat Immunol] 2020 Aug; Vol. 21 (8), pp. 902-913. Date of Electronic Publication: 2020 Jul 20.

Production, Purification and Characterization of Recombinant Biotinylated Phytochrome B for Extracellular Optogenetics.

Autor: Hörner M; Faculty of Biology, University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Yousefi OS; Faculty of Biology, University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany., Schamel WWA; Faculty of Biology, University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.; Center for Chronic Immunodeficiency (CCI), Medical Center Freiburg and Faculty of Medicine, University of Freiburg, Freiburg, Germany., Weber W; Faculty of Biology, University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.

Publikováno v: Bio-protocol [Bio Protoc] 2020 Mar 05; Vol. 10 (5), pp. e3541. Date of Electronic Publication: 2020 Mar 05 (Print Publication: 2020).