Zobrazeno 1 - 10
of 41
pro vyhledávání: '"You-shang Zhang"'
Autor:
Brange, Jens, Whittingham, Jean, Edwards, David, You-Shang, Zhang, Wollmer, Axel, Brandenburg, Dietrich, Dodson, Guy, Finch, John
Publikováno v:
Current Science, 1997 Apr 01. 72(7), 470-476.
Externí odkaz:
https://www.jstor.org/stable/24098450
Autor:
Jiuru Sun, Yi Lu, Jian Fei, Xin Gao, Da-Fu Cui, Jiang Li, You-Shang Zhang, Qi-Chang Xia, Yangbin Huang, Jun Ren, Tao Feng
Publikováno v:
Acta Biochimica et Biophysica Sinica. 38:586-592
In this article we report the production of human proinsulin C-peptide with 31 amino acid residues from a precursor overexpressed in Pichia pastoris. A C-peptide precursor expression plasmid containing nine C-peptide genes in tandem was constructed a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::72d4055d49ce2427605a77c79cf5659b
https://doi.org/10.1111/j.1745-7270.2006.00200.x
https://doi.org/10.1111/j.1745-7270.2006.00200.x
Publikováno v:
Acta Biochimica et Biophysica Sinica. 37:234-240
Monomeric B27 Lys destripeptide insulin (B27 Lys DTrI) was designed and produced from its precursor expressed in Pichia pastoris through tryptic hydrolysis instead of the less efficient tryptic transpeptidation. The monomeric B27 Lys DTrI precursor (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::af66815364ff5b61509a90cf6c851f9e
https://doi.org/10.1111/j.1745-7270.2005.00040.x
https://doi.org/10.1111/j.1745-7270.2005.00040.x
Publikováno v:
Acta Biochimica et Biophysica Sinica. 36:385-389
An aspartame analogue, L-asparaginyl L-3-phenyllactic acid methyl ester was synthesized with aspartic acid replaced by asparagine and peptide bond replaced by ester bond. The aspartic acid of aspartame could be replaced by asparagine as reported in t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a0350a013b130d0e049c94d55b49177d
https://doi.org/10.1093/abbs/36.6.385
https://doi.org/10.1093/abbs/36.6.385
Publikováno v:
The Journal of Peptide Research. 57:188-192
Destetrapeptide insulin (DTI, human insulin with B27–30 removed) was obtained from a monomeric precursor (MIP) expressed in Saccharomyces cerevisiae through tryptic transpeptidation in the presence of synthetic tetrapeptide Gly-Phe-Phe-Tyr. The in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0940b887d265bdc69a0788370f2e0c88
https://doi.org/10.1111/j.1399-3011.2001.00798.x
https://doi.org/10.1111/j.1399-3011.2001.00798.x
Autor:
Ying-Gao Xu, You-Min Feng, Da-Fu Cui, Shi-Yin Yao, Zhen-He Liang, Yan Wang, You-Shang Zhang, Shangquan Zhu, Yue-Hua Tang
Publikováno v:
Biotechnology and Bioengineering. 73:74-79
The methylotrophic yeast Pichia pastoris, which proved successful in producing many heterologous proteins, was used to express an insulin precursor. A transformant with a high copy number of the gene integrated into the chromosome was obtained by the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::98a40e724219169088eae48891b3bb36
https://doi.org/10.1002/1097-0290(20010405)73:1<74::aid-bit1038>3.0.co
https://doi.org/10.1002/1097-0290(20010405)73:1<74::aid-bit1038>3.0.co
Publikováno v:
IUBMB Life. 39:1245-1254
Summary: [B12Thr]human insulin and [Bl2Leu]human insulin were obtained by means of site-directed random mutagenesis. [B12Thr]human insulin retains total biological activity but [B 12Leu]human insulin has much lower biological activity. Receptor bindi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bccd151c587885d02a1cf1c8494af18d
https://doi.org/10.1080/15216549600201442
https://doi.org/10.1080/15216549600201442
Publikováno v:
Retrospect and Prospect of Protein Research. :257-264
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0bfe2ea72040093321e9821693f78219
https://doi.org/10.1142/9789814360425_bmatter
https://doi.org/10.1142/9789814360425_bmatter
Publikováno v:
Retrospect and Prospect of Protein Research. :i-xv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3f964367895a7658afc1dec34e2dffeb
https://doi.org/10.1142/9789814360425_fmatter
https://doi.org/10.1142/9789814360425_fmatter
Publikováno v:
Acta biochimica et biophysica Sinica. 40(2)
B24 and B25 phenylalanines (Phe) play important roles in insulin structure and function. Insulin analogs with B24 Phe or B25 Phe replaced by biphenylalanine (Bip) were prepared by enzymatic semisynthesis. The biological activities were determined by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5d7de388368037daa89da9d19b2e029
https://pubmed.ncbi.nlm.nih.gov/18235975
https://pubmed.ncbi.nlm.nih.gov/18235975