Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Yoshimitsu Nakashima"'
Autor:
Kouichi Mogi, S. Nakajo, H. Akuzawa, Koichiro Kishi, Yoshimitsu Nakashima, Y. Hirota, Shinjiro Mori, Toshihiro Yasuda, Haruo Takeshita, Takashi Nakajima
Publikováno v:
International Journal of Legal Medicine. 114:96-100
A case of accidental death after occupational exposure to an atmosphere containing dichloromethane (DCM) is reported. The concentrations of DCM in the blood and tissues of a 40-year-old man who died while observing an industrial washing machine fille
Autor:
Kouichi Mogi, Koichiro Kishi, Haruo Takeshita, Takashi Nakajima, Y Hanaoka, Toshihiro Yasuda, Osamu Hosomi, Yoshimitsu Nakashima, Shinjiro Mori
Publikováno v:
DNA Sequence. 11:247-255
A 1200-bp cDNA encoding Xenopus laevis deoxyribonuclease I (X. laevis DNase I) was constructed from the total RNA of a X. laevis pancreas using a rapid amplification of cDNA ends method. When the cDNA was transiently transfected into COS-7 cells, the
Autor:
Tamiko Nakajima, Yoshimitsu Nakashima, Toshihiro Yasuda, Yoichi Hanaoka, Osamu Hosomi, Haruo Takeshita, Koichiro Kishi
Publikováno v:
Experimental and Clinical Immunogenetics. 16:117-123
Deoxyribonuclease I (DNase I) was purified from Xenopus laevis pancreas to apparent electrophoretic homogeneity using a series of column chromatographies. The purified enzyme showed a molecular mass of about 36 kDa and maximum activity at pH 7.0–8.
Autor:
Koichiro Kishi, Shinjiro Mori, Toshihiro Yasuda, Yoshimitsu Nakashima, Reiko Iida, Osamu Hosomi, Souichi Tsutsumi, Takashi Nakajima, Haruo Takeshita
Publikováno v:
Annals of Human Genetics. 62:299-305
The structure of the human gene for deoxyribonuclease II (DNase II; EC 3.1.22.1) was determined using several specific primers based on the human DNase II cDNA sequence [Yasuda et al. (1998). J. Biol. Chem. 273, 2610-2616] in a polymerase chain react
Publikováno v:
Applied Surface Science. :352-356
Atomic layer selective deposition of silicon nitride films on the hydrogen-terminated Si surface has been investigated. The film is deposited by alternate supply of SiH2Cl2, and NH3 dissociated by thermal catalytic reaction on a tungsten filament. Th
Autor:
Takashi Nakajima, Haruo Takeshita, Koichiro Kishi, Yoshimitsu Nakashima, Osamu Hosomi, Toshihiro Yasuda
Publikováno v:
Immunological Investigations. 27:145-152
Two novel screening methods, single radial enzyme diffusion and the DNA-cast polyacrylamide gel electrophoresis, for selecting monoclonal antibodies which detect human deoxyribonuclease I (DNase I) enzyme activity are described. The former was adopte
Autor:
Koichiro Kishi, Toshihiro Yasuda, Yoshimitsu Nakashima, Osamu Hosomi, Reiko Iida, Tamiko Nakajima, Haruo Takeshita
Publikováno v:
Journal of Biological Chemistry. 273:2610-2616
A rapid amplification of cDNA ends method, using degenerate oligonucleotides based upon the N-terminal amino acid sequence of human hepatic deoxyribonuclease II (DNase II), allowed a novel cDNA encoding DNase II to be constructed from thyroid gland R
Autor:
Yoshimitsu Nakashima, Osamu Hosomi, Koichiro Kishi, Tamiko Nakajima, Toshihiro Yasuda, Haruo Takeshita
Publikováno v:
IUBMB Life. 42:65-75
Mouse urinary deoxyribonuclease I (DNase I) resembles rat and human DNase Is in terms of its proteochemical and enzymological properties. Furthermore, mouse DNase I was demonstrated to be immunologically closer to the rat than to the human enzyme. A
Autor:
Takasumi Matsuki, Kazumi Sawazaki, Etsuko Tsubota, Yoshimitsu Nakashima, Koichiro Kishi, M Aoyama, Reiko Iida, Toshihiro Yasuda
Publikováno v:
Electrophoresis. 19:2416-2418
A highly sensitive method for detecting deoxyribonucleases (DNases) I and II on an electrophoresed gel is described. A dried agarose film sheet containing DNA as a substrate and a buffer reagent was placed in contact with the gel surface after electr
Autor:
Koichiro Kishi, Osamu Hosomi, Haruo Takeshita, Yoshimitsu Nakashima, Tamiko Nakajima, Toshihiro Yasuda
Publikováno v:
Analytical Biochemistry. 245:255-257