Zobrazeno 1 - 10
of 107
pro vyhledávání: '"Yoshiki Nakashima"'
Autor:
Hirofumi Noguchi, Chika Miyagi-Shiohira, Yoshiki Nakashima, Yuka Onishi, Issei Saitoh, Masami Watanabe
Publikováno v:
Cell Transplantation, Vol 33 (2024)
Recently, we and others generated induced tissue-specific stem/progenitor (iTS/iTP) cells. The advantages of iTS/iTP cells compared with induced pluripotent stem (iPS) cells are (1) easier generation, (2) efficient differentiation, and (3) no teratom
Externí odkaz:
https://doaj.org/article/3ea209ae17a941a8a4ddcc6b8db009c3
Autor:
Hirofumi Noguchi, Yoshiki Nakashima, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara, Issei Saitoh, Chika Miyagi-Shiohira
Publikováno v:
STAR Protocols, Vol 3, Iss 4, Pp 101884- (2022)
Summary: Our recent study demonstrated the generation of induced tissue-specific stem/progenitor (iTS/iTP) cells by the transient overexpression of reprogramming factors combined with tissue-specific selection. Here, we present a protocol to reprogra
Externí odkaz:
https://doaj.org/article/5ef4c8c979234c43ba59dcdacf40ea7c
Autor:
Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara, Hirofumi Noguchi
Publikováno v:
iScience, Vol 25, Iss 10, Pp 105052- (2022)
Summary: Human hepatocytes were transfected with Sendai virus vectors (SeV) expressing OCT3/4, SOX2, KLF4, and C-MYC to produce hepatocyte-derived induced pluripotent stem cells (iPSCs). The messenger RNA (mRNA) expression of undifferentiated markers
Externí odkaz:
https://doaj.org/article/154522eb098b4816be1c681a09bfda0f
Autor:
Yoshiki Nakashima, Hiroki Iguchi, Kenta Takakura, Yuta Nakamura, Kenji Izumi, Naoya Koba, Satoshi Haneda, Masayoshi Tsukahara
Publikováno v:
Cell Transplantation, Vol 31 (2022)
We reported in 2018 that among several extracellular matrices, fibronectin, type I collagen, type IV collagen, laminin I, fibrinogen, and bovine serum albumin, fibronectin is particularly useful for adhesion of porcine pancreatic tissue. Subsequently
Externí odkaz:
https://doaj.org/article/c264d3ba817a40caa64d2ed268d494e7
Autor:
Hirofumi Noguchi, Chika Miyagi-Shiohira, Yoshiki Nakashima, Takao Kinjo, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, A. M. James Shapiro, Tatsuya Kin
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 13, Iss , Pp 243-252 (2019)
We recently demonstrated the generation of mouse induced tissue-specific stem (iTS) cells through transient overexpression of reprogramming factors combined with tissue-specific selection. Here we induced expandable tissue-specific progenitor (iTP) c
Externí odkaz:
https://doaj.org/article/5767112959d040c5ae59cd02073c1b98
Autor:
Hirofumi Noguchi, Koji Sugimoto, Chika Miyagi-Shiohira, Yoshiki Nakashima, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Abstract Calcineurin inhibitors have been used for transplant therapy. However, the inhibition of calcineurin outside the immune system has a number of side effects. We previously developed a cell-permeable inhibitor of NFAT (nuclear factor of activa
Externí odkaz:
https://doaj.org/article/d0a1298d24d44d60b856dbc18ce5f181
Autor:
Yoshiki Nakashima, Saifun Nahar, Chika Miyagi-Shiohira, Takao Kinjo, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Jiro Fujita, Hirofumi Noguchi
Publikováno v:
Stem Cells International, Vol 2019 (2019)
Adipose-derived mesenchymal stem cells (MSC-ATs) are representative cell sources for cell therapy. However, how cell stress resulting from passage influences the MSC-AT protein expression has been unclear. In this study, a protein expression analysis
Externí odkaz:
https://doaj.org/article/d215bb026a3b4e7f8f01f6d49b173386
Autor:
Yoshiki Nakashima, Saifun Nahar, Chika Miyagi-Shiohira, Takao Kinjo, Zensei Toyoda, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Jiro Fujita, Hirofumi Noguchi
Publikováno v:
Cell Transplantation, Vol 27 (2018)
Liquid chromatography using a tandem mass spectrometer (LC-MS/MS) is a method of proteomic analysis. A shotgun analysis by LC-MS/MS comprehensively identifies proteins from tissues and cells with high resolution. The hepatic function of mice with acu
Externí odkaz:
https://doaj.org/article/e632a98fa4094597850d8d9f6fdb4f22
Autor:
Saifun Nahar, Yoshiki Nakashima, Chika Miyagi-Shiohira, Takao Kinjo, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi, Jiro Fujita
Publikováno v:
Stem Cells International, Vol 2018 (2018)
Preservation of adipose tissue before the isolation of cells is one of the most important steps in maintaining the cell viability of adipose tissue-derived mesenchymal stem cells (ADSCs) for clinical use. Hank’s balanced salt solution (HBSS) is one
Externí odkaz:
https://doaj.org/article/2739276d648c4002acd26741bb42ef35
Publikováno v:
Brain Sciences, Vol 8, Iss 9, p 167 (2018)
We searched for drugs that alleviate the reduction of dopaminergic neurons caused by the administration of lipopolysaccharide (LPS) to the substantia nigra of the rat brain. Human milk fat globule-EGF factor 8 protein (MFG-E8) is similar to MFG-E8-S,
Externí odkaz:
https://doaj.org/article/7845879d682645ab878bc9e29e1d5b65