Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Yoshikazu Ichihara"'
Publikováno v:
European Journal of Haematology. 79:59-68
Three Japanese patients demonstrated plasma prekallikrein (PK) deficiency (PKD) after an examination of the proband family line named 'PKD Seki'. A molecular genetic analysis of these PK genes showed homozygous amino acid substitutions Gly104Arg and
Autor:
Ikuya Tsuge, Hiroshi Matsuoka, Shinpei Torii, Tokuichiro Abe, Yoshikazu Ichihara, Yoshikazu Kurosawa, Yoshiro Kamachi
Publikováno v:
European Journal of Immunology. 23:1401-1404
In a previous study (Immunogenetics 1988. 27:330) with Epstein-Barr virus, we established lines of precursor B cells from bone marrow cells of a patient with severe combined immunodeficiency in whom the numbers of B cells and T cells were markedly re
Publikováno v:
Immunology Letters. 33:277-284
The recombination-activating genes RAG-1 and RAG-2 are required for V(D)J DNA rearrangements at loci for immunoglobulin and T cell receptor genes. We isolated the human RAG-2 gene and determined its nucleotide sequence. Mapping analysis of RAG-1 and
Autor:
Kazumasa Miki, Masanobu Obara, Kiyoshi Kurokawa, Chie Furihata, Masashi Matsushima, Takashi Kageyama, Yoshikazu Ichihara, Masae Tatematsu, Masao Tanji, Toshiro Konishi, Takayuki Takahashi, Kenji Takahashi, Hideshi Inoue, Masao Ichinose
Publikováno v:
Biochemical and Biophysical Research Communications. 172:1086-1093
Administration of hydrocortisone to infant rats caused a precocious increase in levels of mucosal pepsinogen and its mRNA together with morphological maturation of pepsinogen-producing cells. The increase in levels of pepsinogen mRNA was induced rapi
Publikováno v:
Molecular immunology. 30(5)
The nucleotide sequence of a 4621 base pair fragment of DNA, from a position upstream of DSQ52 to the S mu region within immunoglobulin heavy-chain gene loci of Suncus murinus was determined. The sequence contained one D gene, three JH genes and an e
Autor:
Yoshikazu Kurosawa, Yoshikazu Ichihara
Publikováno v:
Gene. 130:153-154
More than half of the products of PCR contain an extra A residue at the 3' end, which is the result of the template-independent activity of Taq polymerase. To facilitate cloning of the products of PCR without modification, T vectors, which have a sin
Autor:
Takashi Kageyama, Chie Furihata, Yoshikazu Ichihara, Yasuo Endo, Masao Tanji, Hiroshi Oka, Kenji Takahashi, Kazumasa Miki, Masao Ichinose
Publikováno v:
SEIBUTSU BUTSURI KAGAKU. 32:309-317
Autor:
Ikuya Tsuge, Jun-ichi Okada, Yoshikazu Ichihara, Hiroshi Matsuoka, Hisashi Yasui, Shinpei Torii, Yoshikazu Kurosawa
Publikováno v:
Immunogenetics. 27:330-337
In an attempt to characterize the genes that cause immunodeficiencies such as X-linked agammaglobulinemia (XLA) and severe combined immunodeficiency (SCID) we established precursor B-cell lines by transforming the patients' bone marrow cells with Eps
Publikováno v:
The Journal of Biochemistry. 91:87-95
Membrane-bound neutral proteinase was found in the microsomal fraction of rat skeletal muscle as assayed with heat-denatured casein as a substrate. The enzyme was solubilized from 1 M KCl-washed microsomal fraction by 1 % sodium cholate containing 0.
Publikováno v:
The Journal of Biochemistry. 98:483-492
The total RNAs were extracted from human, swine, rat, and calf gastric mucosae, and translated in vitro in the presence of radiolabeled amino acids using a wheat germ cell-free system. Upon sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophore