Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Yoshifumi Watazu"'
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 64:1352-1358
Mass production of an r-CDH derived from Nocardia species was made possible by gene technology. (Horinouchi et al., Applied and Environmental Microbiology, 57, 1386-1393 (1991)). However, the characteristics of the r-CDH have not been studied in deta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66e5d5a34b5cdc975e227d694357c2df
https://doi.org/10.1271/bbb.64.1352
https://doi.org/10.1271/bbb.64.1352
Autor:
Hiroaki Okabe, Nobuaki Kaneda, Sawao Murao, Yoshifumi Watazu, Katashi Nagamatsu, Yasushi Shirahase
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 58:745-751
A selective inactivating enzyme for the m-subunit of lactate dehydrogenase (LDH) was found in the culture filtrate of Penicillium citrinum KE-1, newly isolated from soil. The enzyme was purified from the culture filtrate by ammonium sulfate fractiona
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e96141460f7c373bfaeffd9d9d1f068
https://doi.org/10.1271/bbb.58.745
https://doi.org/10.1271/bbb.58.745
Autor:
Yasushi Shirahase, Arthur Karmen, Nobuaki Kaneda, Hiroaki Okabe, Yoshifumi Watazu, Yoshinori Uji
Publikováno v:
Journal of Clinical Laboratory Analysis. 7:81-85
We studied a new proteinase K assay method for human serum mitochondrial aspartate aminotransferase. We found that proteinase K showed no inactivation of human mitochondrial aspartate aminotransferase isoenzyme and complete inactivation of cytosolic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dabf9cf7706f950a8c6088f23af6d18a
https://doi.org/10.1002/jcla.1860070202
https://doi.org/10.1002/jcla.1860070202
Publikováno v:
Clinical Chemistry. 38:2193-2196
We devised a method for assaying serum lactate dehydrogenase isoenzyme 1 (LD-1) activity specifically by preincubation with alpha-chymotrypsin and guanidine. Cleavage of phenylalanine bonds in the loop of A and B subunits of LD-3, LD-4, and LD-5 isoe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3555a7ab9cd652e39c566e222282e58e
https://doi.org/10.1093/clinchem/38.11.2193
https://doi.org/10.1093/clinchem/38.11.2193
Publikováno v:
Analytical Sciences. 7:333-335
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ab7e69a3c54e347eeda4d003faba72d8
https://doi.org/10.2116/analsci.7.333
https://doi.org/10.2116/analsci.7.333
Publikováno v:
Clinical Chemistry. 36:687-689
Total mitochondrial aspartate aminotransferase (EC 2.6.1.1), the sum of apo- and holo-mitochondrial aspartate aminotransferase activity in human serum, was measured by using a proteolytic method: inactivation of cytosolic aspartate aminotransferase w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::439e43d422443308d5f3ff256130fab5
https://doi.org/10.1093/clinchem/36.4.687
https://doi.org/10.1093/clinchem/36.4.687
Autor:
Nobuaki Kaneda, Yasushi Shirahase, Hiroyuki Sugiuchi, Yoshinori Uji, Yoshifumi Watazu, Hiroaki Okabe
Publikováno v:
Clinical biochemistry. 23(2)
A new proteolytic measurement of serum mitochondrial aspartate aminotransferase was evaluated using cytosolic aspartate aminotransferase inactivating protease. Some of the proteases, such as, alpha-chymotrypsin, subtilisin and cytosolic aspartate ami
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61e9ef3e4c7ba2e778616d55148364f5
https://pubmed.ncbi.nlm.nih.gov/2197026
https://pubmed.ncbi.nlm.nih.gov/2197026