Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Yoshiaki Seto"'
Publikováno v:
Journal of Bioscience and Bioengineering. 110:392-396
Acyl-CoA thioesterase II (TesB), which catalyzes hydrolysis of acyl-CoAs to free fatty acids and CoA, is involved in 3-hydroxyalkanoic acid production in Escherichia coli. Effects of genetic replacement of tesB with Saccharomyces cerevisiae acyl-CoA
Autor:
Yoshiaki Seto, Shunsaku Ueda, Isamu Maeda, Kiyohito Yagi, Masaya Kawase, Junko Hari, Hitoshi Miyasaka, Yukoh Cheng
Publikováno v:
Biotechnology and Bioengineering. 94:722-729
For production of starch in algal cultures, a growth rate limited by a nutrient is an important factor. Under phototrophic conditions, turbidity must be also paid attention, as the shading effect may affect its productivity. Semi-continuous cultivati
Publikováno v:
IEEJ Transactions on Industry Applications. 125:758-766
Publikováno v:
Bioconjugate Chemistry. 13:303-308
A bioluminescent enzyme immunoassay (BLEIA) for 2',5'-oligoadenylate 5'-triphosphate (pppA2'p5'A2'Ap5'A, 2-5A) was developed using a conjugate of 2-5A and firefly luciferase as a probe. The conjugate was prepared from a 2-5A derivative bearing a thio
Publikováno v:
Analytica Chimica Acta. 429:19-26
A bioluminescent enzyme immunoassay (BLEIA) of thyroid-stimulating hormone (TSH) using biotinylated firefly luciferase as a labelling enzyme for an antibody was developed. This immunoassay, of which method was one step sandwich method, had high sensi
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 3:2883-2886
A novel bi-functional fluorescent labelling agent derived from 5-hydroxy-1-naphthalene sulfonic acid and tris-2-aminoethyl)amine was synthesized and incorporated into the 5′-terminus of a 15-mer oligoDNA through phosphoroamidate linkage. The labell
Publikováno v:
The Journal of biological chemistry. 281(17)
The role of the Saccharomyces cerevisae peroxisomal acyl-coenzyme A (acyl-CoA) thioesterase (Pte1p) in fatty acid beta-oxidation was studied by analyzing the in vitro kinetic activity of the purified protein as well as by measuring the carbon flux th
Publikováno v:
Luminescence : the journal of biological and chemical luminescence. 16(4)
A bioluminescent enzyme immunoassay (BLEIA) for prostate-specific antigen (PSA) using biotinylated firefly luciferase-labelled antibody was developed. PSA is an important marker for the diagnosis and management of prostate cancer. Our BLEIA for PSA,
Publikováno v:
JOURNAL OF THE ILLUMINATING ENGINEERING INSTITUTE OF JAPAN. 82:174-174
Publikováno v:
Journal of the Chemical Society, Chemical Communications. :1377
A 15-mer DNA labelled with a novel multifunctional fluorescent agent bearing acridine and fluorescein moieties is synthesized and the intensity of fluorescein-based fluorescence detected by the excitation of acridine is shown to be strongly affected