Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Yongya Li"'
Amplified detection of nucleic acids and proteins using an isothermal proximity CRISPR Cas12a assay†
Publikováno v:
Chemical Science
Herein, we describe an isothermal proximity CRISPR Cas12a assay that harnesses the target-induced indiscrimitive single-stranded DNase activity of Cas12a for the quantitative profiling of gene expression at the mRNA level and detection of proteins wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f723ea9e5302598af953e20a92d8a785
http://europepmc.org/articles/PMC8179305
http://europepmc.org/articles/PMC8179305
Publikováno v:
Chemical Communications. 56:3536-3538
Herein, we introduce a sequence-specific plasmonic loop-mediated isothermal amplification (LAMP) assay with dual, complementary color readouts enabled by CRISPR Cas12a. Using this assay, any double-stranded LAMP amplicon containing a 5'-TTN PAM seque
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2469d95e980e75fa9d32a1a04ea2896b
https://doi.org/10.1039/d0cc00397b
https://doi.org/10.1039/d0cc00397b
Autor:
Yongya Li, Feng Li, Rong-Xing Zhou, Fangfang Chen, Xiandeng Hou, Sean D. Mason, Junbo Chen, Peng Yang, Juewen Liu
Publikováno v:
Analytical chemistry. 92(4)
Colloidal nanoparticle biosensors capable of on-particle biocatalysis are powerful tools for amplified detection of biomolecules. The development and practical uses of such concentric amplifiers can be complicated because of the on-particle biorecogn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c5ee0f1a69c4fb4f63f77517040a1a1
https://pubmed.ncbi.nlm.nih.gov/31957445
https://pubmed.ncbi.nlm.nih.gov/31957445
Publikováno v:
Chemical Science. 9:6434-6439
Single nucleotide variants (SNVs) are important both clinically and biologically because of their profound biological consequences. Herein, we engineered a nicking endonuclease-powered three dimensional (3D) DNA nanomachine for discriminating SNVs wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::27c1acfcefc532bec10518e1d50f540f
https://doi.org/10.1039/c8sc02761g
https://doi.org/10.1039/c8sc02761g
Publikováno v:
Analytical chemistry. 91(18)
Herein, we described a novel plasmonic CRISPR Cas12a assay for the visual, colorimetric detection of grapevine viral infections. Our assay generates rapid and specific colorimetric signals for nucleic acid amplicons by combining the unique target-ind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8beaa6fc63ab8349b60210c4bc060c1c
https://pubmed.ncbi.nlm.nih.gov/31478642
https://pubmed.ncbi.nlm.nih.gov/31478642
Publikováno v:
ACS nano. 13(7)
Herein, we report a bottom-up approach to assemble a series of stochastic DNA walkers capable of probing dynamic interactions occurring at the bio-nano interface. We systematically investigated the impact of varying interfacial factors, including int
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c6547ce09e0665657828ea1a4a92752
https://pubmed.ncbi.nlm.nih.gov/31241883
https://pubmed.ncbi.nlm.nih.gov/31241883
Publikováno v:
ACS Nano; 7/23/2019, Vol. 13 Issue 7, p8106-8113, 8p