Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Yonghua Taylor Zhang"'
Autor:
Bingchuan Wei, Xuan Gao, Lance Cadang, Saeed Izadi, Peilu Liu, Hui-Min Zhang, Elizabeth Hecht, Jeongsup Shim, Gordon Magill, Juan Rincon Pabon, Lu Dai, Wilson Phung, Elaine Lin, Christopher Wang, Kevin Whang, Sean Sanchez, Jose Oropeza Jr, Julien Camperi, Jennifer Zhang, Wendy Sandoval, Yonghua Taylor Zhang, Guoying Jiang
Publikováno v:
mAbs, Vol 13, Iss 1 (2021)
Fc galactosylation is a critical quality attribute for anti-tumor recombinant immunoglobulin G (IgG)-based monoclonal antibody (mAb) therapeutics with complement-dependent cytotoxicity (CDC) as the mechanism of action. Although the correlation betwee
Externí odkaz:
https://doaj.org/article/bdd3b615610e438ebfe86ec19cb0df05
Publikováno v:
Analytical Chemistry. 91:15360-15364
Conventionally, hydrophobic interaction chromatography (HIC) uses mobile phases with high salt concentration that are not compatible with mass spectrometry (MS). Here we describe development of an HIC method coupled with MS detection (HIC-MS) utilizi
Autor:
Juan Pablo Rincon Pabon, Jennifer Zhang, Peilu Liu, Saeed Izadi, Lu Dai, Guoying Jiang, Yonghua Taylor Zhang, Julien Camperi, Wendy Sandoval, Gordon Magill, Sean Sanchez, Hui-Min Zhang, Xuan Gao, Jose Oropeza, Elaine Lin, Elizabeth S. Hecht, Bingchuan Wei, Lance Cadang, Kevin Whang, Christopher Wang, Jeongsup Shim, Wilson Phung
Publikováno v:
mAbs
article-version (VoR) Version of Record
article-version (VoR) Version of Record
Fc galactosylation is a critical quality attribute for anti-tumor recombinant immunoglobulin G (IgG)-based monoclonal antibody (mAb) therapeutics with complement-dependent cytotoxicity (CDC) as the mechanism of action. Although the correlation betwee
Autor:
Mansour Jazayri, John B. Briggs, Hongbin Liu, Zane Liu, Qian Cai, Charlene Li, Milady R. Niñonuevo, Weitao Jia, Yi Yang, Bingchuan Wei, David Fulchiron, Yonghua Taylor Zhang, Justin Jeong
Publikováno v:
Journal of pharmaceutical and biomedical analysis. 189
Free thiols, or unpaired cysteines, are important product quality attributes in the therapeutic proteins due to their potential impact on the protein structure, bioactivity and stability. While many free thiol quantitation methods were developed for
Publikováno v:
TrAC Trends in Analytical Chemistry. 101:34-42
Reactive impurities in excipients can cause drug product degradation or protein modification even at trace levels, and thus impact drug stability and quality. It is critical to understand the potential impact of these impurities during development in
Publikováno v:
J Chromatogr A
Reversed-phase liquid chromatography (RPLC) has been commonly used in IgG2 disulfide isoforms analysis. Recently, the columns packed with large pore superficially porous particles (SPP) have become available commercially. This work explores the appli
Autor:
Yonghong Chen, X. Christopher Yu, Ying Cheng, Matthew T. Chen, Yonghua Taylor Zhang, Lisa C. Patterson, Braydon L. Burgess
Publikováno v:
Analytical Biochemistry. 519:8-14
Measurement of free thiols in antibody therapeutics is important for product development and assessment of critical quality attributes. Earlier studies demonstrated fast separation of free thiol variants of IgG1 using reversed-phase high performance
Publikováno v:
Journal of Pharmaceutical and Biomedical Analysis. 109:52-61
Size-exclusion chromatography (SEC) is an important mode of separation used in monoclonal antibody (mAb) characterization and quality control. SEC separates mAbs into three major species: high molecular weight species, main peak (predominantly monome
Publikováno v:
Journal of Chromatography B. 965:65-71
Deamidation is one of the most common degradation pathways for proteins and frequently occurs at "hot spots" with Asn-Gly, Asn-Ser or Asn-Thr sequences. Occasionally, deamidation may occur at other motifs if the local protein structure can participat
Publikováno v:
Journal of Pharmaceutical Sciences. 102:1712-1723
The deamidation of asparagine into aspartate and isoaspartate moieties is a major pathway for the chemical degradation of monoclonal antibodies (mAbs). It can affect the shelf life of a therapeutic antibody that is not formulated or stored appropriat