Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Yong-Feng Jin"'
MicroRNAs (miRNAs) constitute a novel, extensive class of small RNAs (approximately 21 nucleotides), and play important gene-regulation roles during growth and development in various organisms. Here we conducted a homology search to identify homologs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b63e50673d23e3fe744c7cd5d7b19779
https://europepmc.org/articles/PMC1599803/
https://europepmc.org/articles/PMC1599803/
Publikováno v:
Yi chuan = Hereditas. 26(4)
TP-PCR,a method developed for fusion gene construction without the use of endonuclease and ligase, was performed to construct a fused fpg gene. The TP-PCR reaction system contained three primers and two templates and resulting PCR product, fused fpg
Autor:
Yong-Feng Jin, Teng-Fei Bian
Publikováno v:
Acta biochimica et biophysica Sinica. 36(7)
A novel pollen-specific full-length cDNA clone PSG076 was isolated using suppression subtractive hybridization and 5'/3' RACE techniques. PSG076 was shown to exhibit multi-site polyadenylation by sequencing the 3' ends of the cDNAs. At least six tran
Publikováno v:
Acta biochimica et biophysica Sinica. 36(5)
The mutated osteoprotegerin (OPG-372) gene was inserted into the baculovirus transfer vector pBacPAK8, and the recombinant plasmid was co-transfected with linearized Bm-BacPAK6 virus DNA into BmN cells, then homologous recombination occurred inside t
Publikováno v:
Journal of microbiological methods. 56(3)
A novel tri-primer polymerase chain reaction method (TP-PCR) was developed for the construction of a fused fpg gene, in which no endonuclease and ligase were used. Instead, two templates and three specifically designed primers were applied. Results s
Publikováno v:
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 18(4)
Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected
Publikováno v:
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 34(4)
Angiostatin (k1-3) gene was inserted into Bombyx mori baculovirus transfer vector pBacPAK8 and cotransfected with lineared DNA of Bm-BacPAK6 virus into BmN cells. The homologous recombination occurred inside the cells, and the recombinant virus BacPA
Publikováno v:
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 32(3)
VP2 cDNA gene of the infectious bursal disease virus HZ96 strain, encoding a major host-protective antigen, was cloned into baculovirus transfer vector pBacPAK8, resulting in a recombinant transfer vector pBacPAK-VP2. The vector pBacPAK-VP2 and linea
Publikováno v:
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 18(1)
A recombinant transfer vector, pBacIL-11, containing hIL-11 cDNA of 546 nucleotides lacking leader sequence was constructed and co-transfected into BmN cells with linearized BmBacPAK(modified BmNPV) DNA for construction of a recombinant baculovirus c
Autor:
Jian Chen, Zuo-Ming Nie, Zheng-Bing Lü, Cheng-Gang Zhu, Cheng-Zhi Xu, Yong-Feng Jin, Xiang-Fu Wu, Yao-Zhou Zhang
Publikováno v:
Applied Biochemistry & Biotechnology; Apr2007, Vol. 141 Issue 1, p149-160, 12p