Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Yoneko Tobita"'
Autor:
Keiko Higuchi, Syoko Ebihara, Mashahiro Ikeda, Yumiko Hosaka, Toshiko Mochizuki, Hironobu Okazaki, Akihiro Usami, Yukiko Ishikawa, Seiji Ishiyama, Hidekazu Nagaya, Teruyuki Koyama, Yoshimi Asano, Takeshi Yamamoto, Chiaki Enomoto, Mutsumi Sugai, Yoneko Tobita
Publikováno v:
Journal of Biochemistry. 149:219-227
Using a hybrid baculovirus system, we compared the expression of 45 recombinant proteins from six categories using two models: silkworm (larvae and pupae) and an Sf9 cell line. A total of 45 proteins were successfully expressed; preparation of hybrid
Autor:
Toshimichi Kanaya, Yuichi Yokomizo, Hidekazu Nagaya, Yoneko Tobita, Takashi Onodera, Shigeki Inumaru
Publikováno v:
Biotechnology Letters. 30:41-45
Recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF) was produced by the baculovirus-silkworm expression system. It was purified to 98% by (NH(4))(2)SO(4), followed by a three-step column chromatography with silica gel, ion
Autor:
Yoneko Tobita, Ahmed F. Halim, Osama B. Abdel-Halim, Koichi Takeya, Hidekazu Nagaya, Toshiki Nagae, Hideji Itokawa
Publikováno v:
ChemInform. 28
Eighteen dammarane-type triterpenes were obtained from the whole plant of Cleome africana by means of cytotoxic bioassay-directed fractionation. Twelve of them were novel compounds whose structures were elucidated by various spectroscopic methods.
Autor:
Yuichi Yokomizo, Hiroki Kaki, Hitomi Takahashi, Masashi Takahashi, Hidekazu Nagaya, Shigeki Inumaru, Toshimichi Kanaya, Yoneko Tobita
Publikováno v:
The Journal of veterinary medical science. 66(11)
We developed a procedure for the large-scale purification of bovine interferon-tau (boIFN-tau) by means of a silkworm-baculovirus gene expression system. Recombinant boIFN-tau (rboIFN-tau) was efficiently produced in the silkworm infected with boIFN-
Autor:
Hidekazu Nagaya, Hideji Itokawa, Koichi Takeya, Osama B. Abdel-Halim, Ahmed F. Halim, Yoneko Tobita, Toshiki Nagae
Publikováno v:
Phytochemistry. 44(6)
Eighteen dammarane-type triterpenes were obtained from the whole plant of Cleome africana by means of cytotoxic bioassay-directed fractionation. Twelve of them were novel compounds whose structures were elucidated by various spectroscopic methods.