Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Yoneko Tobita"'
Autor:
Keiko Higuchi, Syoko Ebihara, Mashahiro Ikeda, Yumiko Hosaka, Toshiko Mochizuki, Hironobu Okazaki, Akihiro Usami, Yukiko Ishikawa, Seiji Ishiyama, Hidekazu Nagaya, Teruyuki Koyama, Yoshimi Asano, Takeshi Yamamoto, Chiaki Enomoto, Mutsumi Sugai, Yoneko Tobita
Publikováno v:
Journal of Biochemistry. 149:219-227
Using a hybrid baculovirus system, we compared the expression of 45 recombinant proteins from six categories using two models: silkworm (larvae and pupae) and an Sf9 cell line. A total of 45 proteins were successfully expressed; preparation of hybrid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e3720caf8d6d51642c1bd5bc6eb450f
https://doi.org/10.1093/jb/mvq138
https://doi.org/10.1093/jb/mvq138
Autor:
Toshimichi Kanaya, Yuichi Yokomizo, Hidekazu Nagaya, Yoneko Tobita, Takashi Onodera, Shigeki Inumaru
Publikováno v:
Biotechnology Letters. 30:41-45
Recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF) was produced by the baculovirus-silkworm expression system. It was purified to 98% by (NH(4))(2)SO(4), followed by a three-step column chromatography with silica gel, ion
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28c3a4894a78a9fb4c6f1cd47615efd3
https://doi.org/10.1007/s10529-007-9506-3
https://doi.org/10.1007/s10529-007-9506-3
Autor:
Yoneko Tobita, Ahmed F. Halim, Osama B. Abdel-Halim, Koichi Takeya, Hidekazu Nagaya, Toshiki Nagae, Hideji Itokawa
Publikováno v:
ChemInform. 28
Eighteen dammarane-type triterpenes were obtained from the whole plant of Cleome africana by means of cytotoxic bioassay-directed fractionation. Twelve of them were novel compounds whose structures were elucidated by various spectroscopic methods.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a20ea3ce0af1f0e33299bf30868c376a
https://doi.org/10.1002/chin.199723220
https://doi.org/10.1002/chin.199723220
Autor:
Yuichi Yokomizo, Hiroki Kaki, Hitomi Takahashi, Masashi Takahashi, Hidekazu Nagaya, Shigeki Inumaru, Toshimichi Kanaya, Yoneko Tobita
Publikováno v:
The Journal of veterinary medical science. 66(11)
We developed a procedure for the large-scale purification of bovine interferon-tau (boIFN-tau) by means of a silkworm-baculovirus gene expression system. Recombinant boIFN-tau (rboIFN-tau) was efficiently produced in the silkworm infected with boIFN-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::014cb950da8fbda84ed51fbc92ed5955
https://pubmed.ncbi.nlm.nih.gov/15585954
https://pubmed.ncbi.nlm.nih.gov/15585954
Autor:
Hidekazu Nagaya, Hideji Itokawa, Koichi Takeya, Osama B. Abdel-Halim, Ahmed F. Halim, Yoneko Tobita, Toshiki Nagae
Publikováno v:
Phytochemistry. 44(6)
Eighteen dammarane-type triterpenes were obtained from the whole plant of Cleome africana by means of cytotoxic bioassay-directed fractionation. Twelve of them were novel compounds whose structures were elucidated by various spectroscopic methods.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4aaa28f92c7343aed0d5482650c1a9bd
https://pubmed.ncbi.nlm.nih.gov/9101665
https://pubmed.ncbi.nlm.nih.gov/9101665
Autor:
Usami, Akihiro1, Ishiyama, Seiji1, Enomoto, Chiaki1, Okazaki, Hironobu1, Higuchi, Keiko1, Ikeda, Mashahiro1, Yamamoto, Takeshi1, Sugai, Mutsumi1, Ishikawa, Yukiko1, Hosaka, Yumiko1, Koyama, Teruyuki1, Tobita, Yoneko1, Ebihara, Syoko1, Mochizuki, Toshiko1, Asano, Yoshimi1, Nagaya, Hidekazu1 h.nagaya@katakura.co.jp
Publikováno v:
Journal of Biochemistry. Feb2011, Vol. 149 Issue 2, p219-227. 9p. 1 Diagram, 1 Chart, 7 Graphs.
Autor:
Nagaya, Hidekazu, Kanaya, Toshimichi, Tobita, Yoneko, Yokomizo, Yuichi, Inumaru, Shigeki, Onodera, Takashi
Publikováno v:
Biotechnology Letters; Jan2008, Vol. 30 Issue 1, p41-45, 5p, 2 Black and White Photographs, 1 Diagram, 2 Charts
Autor:
Katsumi Maenaka, Enoch Y. Park
While silk derived from silkworm has been of economic importance for centuries, more recently silkworm has been found to have utility in biomedicine. This has attracted attention for expressing eukaryotic recombinant proteins, which require post-tran
