Improved transformation efficiency in Mycoplasma hominis enables disruption of the MIB–MIP system targeting human immunoglobulins

Autor: Jennifer Guiraud, Chloé Le Roy, Fabien Rideau, Pascal Sirand-Pugnet, Carole Lartigue, Cécile Bébéar, Yonathan Arfi, Sabine Pereyre

Publikováno v: Microbiology Spectrum, Vol 11, Iss 5 (2023)

ABSTRACT The pathogenicity of Mycoplasma hominis is poorly understood, mainly due to the absence of efficient genetic tools. A polyethylene glycol-mediated transformation protocol was recently developed for the M. hominis reference strain M132 using

Externí odkaz: https://doaj.org/article/3521865f01df493bbfe56bee26884b12

Beware of Mycoplasma Anti-immunoglobulin Strategies

Autor: Yonathan Arfi, Carole Lartigue, Pascal Sirand-Pugnet, Alain Blanchard

Publikováno v: mBio, Vol 12, Iss 6 (2021)

ABSTRACT Mycoplasmas are small, genome-reduced bacteria. They are obligate parasites that can be found in a wide range of host species, including the majority of livestock animals and humans. Colonization of the host can result in a wide spectrum of

Externí odkaz: https://doaj.org/article/3ee3a701de054a9a88d21aba259fb6cd

Evolution of the CRISPR-Cas9 defence system following a bacterial host shift

Autor: Thomas Ipoutcha, Iason Tsarmpopoulos, Géraldine Gourgues, Vincent Baby, Paul Dubos, Geoffrey E. Hill, Andrea Dowling, Yonathan Arfi, Carole Lartigue, Patricia Thebault, Camille Bonneaud, Pascal Sirand-Pugnet

CRISPR-Cas systems are rapidly evolving bacterial defences targeting phages and mobile genetic elements. How these defences evolve in novel host environments remains, however, unknown. We address this question usingMycoplasma gallisepticum, a bacteri

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9386db8015950ed1c3103607a8213c34
https://doi.org/10.1101/2023.03.14.532377

Unblocking genome editing of major animal mycoplasmas using CRISPR/Cas9 base editor systems

Autor: Thomas Ipoutcha, Fabien Rideau, Geraldine Gourgues, Yonathan Arfi, Carole Lartigue, Alain Blanchard, Pascal Sirand-Pugnet

Mycoplasmas are minimal bacteria that infect humans, wildlife, and most economically important livestock species. Mycoplasma infections cause a large range of chronic inflammatory diseases, eventually leading to death in some animals. Due to the lack

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::70b6d94e2d4ee0eefd57be78784dff86
https://doi.org/10.1101/2022.03.09.483585

The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction

Autor: Pierre Nottelet, Carole Lartigue, Rémi Fronzes, Géraldine Gourgues, Laure Bataille, Esther Marza, Robin Anger, Yonathan Arfi, Pascal Sirand-Pugnet

Publikováno v: Science Advances
'Science Advances ', vol: 7, pages: eabf2403-1-eabf2403-15 (2021)
Science Advances, American Association for the Advancement of Science (AAAS), 2021, 7 (10), pp.eabf2403. ⟨10.1126/sciadv.abf2403⟩
Science Advances, 2021, 7 (10), pp.eabf2403. ⟨10.1126/sciadv.abf2403⟩

MIB and MIP bind to antibodies and disrupt the antigen binding site to promote the dissociation of antibody-antigen interactions.
Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7d0a8dd0a8e6369987c5545f4fb9cab
https://pubmed.ncbi.nlm.nih.gov/33674316