A possible universal role for mRNA secondary structure in bacterial translation revealed using a synthetic operon

Autor: Yonatan Chemla, Michael Peeri, Mathias Luidor Heltberg, Jerry Eichler, Mogens Høgh Jensen, Tamir Tuller, Lital Alfonta

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)

The mechanisms for regulating translation re-initiation in bacteria remain poorly understood. Here, the authors screened a library of synthetic operons and identified a ribosome termination structure that modulates re-initiation efficiency and which

Externí odkaz: https://doaj.org/article/f945cec54ed844a3bf6360085372dfe1

Simplified methodology for a modular and genetically expanded protein synthesis in cell-free systems

Autor: Yonatan Chemla, Eden Ozer, Michael Shaferman, Ben Zaad, Rambabu Dandela, Lital Alfonta

Publikováno v: Synthetic and Systems Biotechnology, Vol 4, Iss 4, Pp 189-196 (2019)

Genetic code expansion, which enables the site-specific incorporation of unnatural amino acids into proteins, has emerged as a new and powerful tool for protein engineering. Currently, it is mainly utilized inside living cells for a myriad of applica

Externí odkaz: https://doaj.org/article/856329b342164fd4a59955cdb76854cc

Mitochondrial 16S rRNA Is Methylated by tRNA Methyltransferase TRMT61B in All Vertebrates.

Autor: Dan Bar-Yaacov, Idan Frumkin, Yuka Yashiro, Takeshi Chujo, Yuma Ishigami, Yonatan Chemla, Amit Blumberg, Orr Schlesinger, Philipp Bieri, Basil Greber, Nenad Ban, Raz Zarivach, Lital Alfonta, Yitzhak Pilpel, Tsutomu Suzuki, Dan Mishmar

Publikováno v: PLoS Biology, Vol 14, Iss 9, p e1002557 (2016)

The mitochondrial ribosome, which translates all mitochondrial DNA (mtDNA)-encoded proteins, should be tightly regulated pre- and post-transcriptionally. Recently, we found RNA-DNA differences (RDDs) at human mitochondrial 16S (large) rRNA position 9

Externí odkaz: https://doaj.org/article/b0b5d25bb08e4ce4a5914113f537bc16

A possible universal role for mRNA secondary structure in bacterial translation revealed using a synthetic operon

Autor: Michael Peeri, Lital Alfonta, Jerry Eichler, Mathias L. Heltberg, Yonatan Chemla, Mogens H. Jensen, Tamir Tuller

Publikováno v: Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Chemla, Y, Peeri, M, Heltberg, M L, Eichler, J, Jensen, M H, Tuller, T & Alfonta, L 2020, ' A possible universal role for mRNA secondary structure in bacterial translation revealed using a synthetic operon ', Nature Communications, vol. 11, no. 1, 4827 . https://doi.org/10.1038/s41467-020-18577-4

In bacteria, translation re-initiation is crucial for synthesizing proteins encoded by genes that are organized into operons. The mechanisms regulating translation re-initiation remain, however, poorly understood. We now describe the ribosome termina

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d618b19437ddb72c3d5f2e74e943b0a6
http://europepmc.org/articles/PMC7518266

Context effects of genetic code expansion by stop codon suppression

Autor: Yonatan Chemla, Eden Ozer, Itay Algov, Lital Alfonta

Publikováno v: Current opinion in chemical biology. 46

Genetic code expansion enables the incorporation of unnatural amino acids into proteins thereby augmenting their physical and chemical properties. This is achieved by the reassignment of codons from their original sense to incorporate unnatural amino

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8e7633f2486ed9e6cd239c031849a39
https://pubmed.ncbi.nlm.nih.gov/30064064