Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Yohichi Mochizuki"'
Publikováno v:
Scopus-Elsevier
ResearcherID
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Proliferation of adult rat hepatocytes is observed in serum-free Dulbecco's modified Eagle's medium (DMEM) supplemented with 10 mmol/L nicotinamide and 10 ng/mL epidermal growth factor (EGF). The proliferating cells are mainly mononucleate and form s
Autor:
Shigeki Miyamoto, Hiroshi Takeda, Keisuke Harada, Shinichi Sugimoto, Koichi Hirata, Yohichi Mochizuki, Shinichiro Ikeda, Toshihiro Mitaka
Publikováno v:
Journal of Hepatology. 39:716-723
Background/Aims : Hybrid bioartificial liver devices supporting a large mass of metabolically active hepatocytes are thought to be necessary for the successful treatment of patients with severe acute liver failure. However, it is very difficult to ob
Autor:
Keisuke Harada, Fumihiko Sato, Toshihiro Mitaka, Yohichi Mochizuki, Koichi Hirata, Shinichiro Ikeda
Publikováno v:
Surgery. 133:101-109
Surgeons sometimes encounter hyperbilirubinemia without mechanical obstruction of the biliary tree postoperatively. Many of these patients have bacterial infections and endotoxemia. Kupffer's cells stimulated by endotoxin secrete inflammatory cytokin
Autor:
Shinichi Sugimoto, Yohichi Mochizuki, Toshihiro Mitaka, Keisuke Harada, Shinichiro Ikeda, Iwao Ikai, Yoshio Yamaoka
Publikováno v:
Journal of Cellular Biochemistry. 87:16-28
Small hepatocytes (SHs), which are known to be hepatic progenitor cells, were isolated from an adult rat liver. SHs in a colony sometimes change their shape from small to large and from flat to rising/piled-up. The aim of the present study is to clar
Autor:
Masao Yamamoto, Yasuo Kokai, Hideki Chiba, Yohichi Mochizuki, Norimasa Sawada, Takashi Kojima
Publikováno v:
Experimental Cell Research. 263:193-201
On freeze-fracture replicas, gap junctions are frequently colocalized with tight junctions. In this study, to elucidate the relationship between gap- and tight-junction proteins, we investigated the localization of gap-junction proteins Cx32 and Cx26
Autor:
Shinichiro Ikeda, Koichi Hirata, Nagato Higaki, W.H. Lamers, Fumihiko Sato, S.I. Sugimoto, Toshihiro Mitaka, Yohichi Mochizuki
Publikováno v:
Cell and Tissue Research, 306(3), 467-471. Springer Verlag
Cell and tissue research, 306(3), 467-471. Springer Verlag
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Cell and tissue research, 306(3), 467-471. Springer Verlag
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Expression of carbamoylphosphate synthetase I and glutamine synthetase in hepatic organoids reconstructed by rat small hepatocytes and hepatic nonparenchymal cells.Mitaka T, Sato F, Ikeda SI, Sugimoto SI, Higaki N, Hirata K, Lamers WH, Mochizuki Y.De
Publikováno v:
Artificial Organs. 24:271-277
To develop an effective hybrid bioartificial liver (BAL) device, the material of the scaffold is very important to support hepatocytes that have both growth ability and hepatic differentiated functions. In this study we used paper (Kimwipe, Kimberly-
Autor:
Elliot L. Hertzberg, Takashi Kojima, Marcia Urban, Norimasa Sawada, Hideki Chiba, Masao Yamamoto, Gang Hong Lee, David C. Spray, Yohichi Mochizuki, Yasuo Kokai
Publikováno v:
Biochemical and Biophysical Research Communications. 266:222-229
Small gap junction plaques are associated with tight junction strands in some cell types including hepatocytes and it is thought that they may be closely related to tight junctions and the establishment of cell polarity. In order to examine roles of
Publikováno v:
Hepatology. 29:111-125
Hepatic cells isolated from an adult rat liver, consisting of small hepatocytes (SHs), mature hepatocytes (MHs), liver epithelial cells (LECs), Kupffer cells, sinusoidal endothelial cells, and stellate cells, were cultured in a medium supplemented wi
Publikováno v:
Medical Electron Microscopy. 31:169-176
Occludin, an integral membrane protein, is a candidate for forming the functional intercellular seal of the tight junction. In the present study, we examined the changes of occludin expression and tight junction strand in the cultured hepatocytes. In