Výsledky vyhledávání

NOPCHAP1 is a PAQosome cofactor that helps loading NOP58 on RUVBL1/2 during box C/D snoRNP biogenesis

Autor: Franck Vandermoere, Tiago M. Bandeiras, Ana C. F. Paiva, Edouard Bertrand, Bruno Charpentier, Xavier Manival, Céline Verheggen, Marie-Eve Chagot, Pedro M. F. Sousa, Marc Quinternet, Marie-Cécile Robert, Philippe Fort, Paulo E. Santo, Jonathan Bizarro, Yoann Abel

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, In press, 49 (2), pp.1094-1113. ⟨10.1093/nar/gkaa1226⟩
Nucleic Acids Research, 2021, 49 (2), pp.1094-1113. ⟨10.1093/nar/gkaa1226⟩

The PAQosome is a large complex composed of the HSP90/R2TP chaperone and a prefoldin-like module. It promotes the biogenesis of cellular machineries but it is unclear how it discriminates closely related client proteins. Among the main PAQosome clien

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa3353ae141945db90b5fe30d422a6fb
https://doi.org/10.1093/nar/gkaa1226

Zobrazit plný text záznamu

The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity

Autor: Yoann Abel, Christophe Charron, Camille Virciglio, Valérie Bourguignon-Igel, Marc Quinternet, Marie-Eve Chagot, Marie-Cécile Robert, Céline Verheggen, Christiane Branlant, Edouard Bertrand, Xavier Manival, Bruno Charpentier, Mathieu Rederstorff

Publikováno v: Nucleic acids research. 50(4)

MicroRNAs silence mRNAs by guiding the RISC complex. RISC assembly occurs following cleavage of pre-miRNAs by Dicer, assisted by TRBP or PACT, and the transfer of miRNAs to AGO proteins. The R2TP complex is an HSP90 co-chaperone involved in the assem

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c98f0cfd2ca86906f8fa821a360a434d
https://pubmed.ncbi.nlm.nih.gov/35150569

Zobrazit plný text záznamu

SnoRNAs and the emerging class of sdRNAs: Multifaceted players in oncogenesis

Autor: Yoann Abel, Mathieu Rederstorff

Publikováno v: Biochimie
Biochimie, Elsevier, 2019, 164, pp.17-21. ⟨10.1016/j.biochi.2019.05.006⟩

Small nucleolar RNAs are generally involved in the modification of target ribosomal RNAs. Other possible functions recently emerged with the discovery of the novel class of snoRNA-derived RNAs or sdRNAs. Since then, additional data has revealed the i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2f3bc0c3f698021d61a674c215f6c77
https://doi.org/10.1016/j.biochi.2019.05.006

Zobrazit plný text záznamu

RNA Precipitation

Autor: Valérie Bourguignon-Igel, Yoann Abel, Mathieu Rederstorff

Publikováno v: Methods in Molecular Biology; Small non-coding RNAs: Methods and Protocols
Methods in Molecular Biology; Small non-coding RNAs: Methods and Protocols, pp.11-16, 2021, ⟨10.1007/978-1-0716-1386-3_2⟩
Methods in Molecular Biology ISBN: 9781071613856

International audience

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63216b0269de52ac274f33890c879727
https://hal.univ-lorraine.fr/hal-03190751

Zobrazit plný text záznamu

Stem-Loop qRT-PCR-Based Quantification of miRNAs

Autor: Yoann, Abel, Mathieu, Rederstorff

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2300

Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f12ae9d5da562a0141cecbde381bb081
https://pubmed.ncbi.nlm.nih.gov/33792871

Zobrazit plný text záznamu

Gene Reporter Assays to Study miRNA Function

Autor: Yoann Abel, Mathieu Rederstorff

Publikováno v: Methods in Molecular Biology; Small non-coding RNAs: Methods and Protocols
Methods in Molecular Biology; Small non-coding RNAs: Methods and Protocols, pp.119-131, 2021, ⟨10.1007/978-1-0716-1386-3_12⟩
Methods in Molecular Biology ISBN: 9781071613856

This chapter describes one of the most reliable quantitative assays to test the silencing of a possible target gene by a specific miRNA using a luciferase reporter gene. The experimental procedure first consists in cloning both the wild-type and muta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ad558ce6dbb3a2dbffd94f706d62d29
https://hal.univ-lorraine.fr/hal-03190757

Zobrazit plný text záznamu

Regulatory Non-Coding RNAs: An Overview

Autor: Camille, Virciglio, Yoann, Abel, Mathieu, Rederstorff

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2300

The discovery of new classes of non-coding RNAs has always been preceded or accompanied by technological breakthroughs, and these outstanding progresses in transcriptomics approaches enabled to regularly add new members to the list. From the first de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::057c39868c005f9116a62005b38943ae
https://pubmed.ncbi.nlm.nih.gov/33792866

Zobrazit plný text záznamu

Gene Reporter Assays to Study miRNA Function

Autor: Yoann, Abel, Mathieu, Rederstorff

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2300

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::50ce6259beb540b00f185a975b0a7502
https://pubmed.ncbi.nlm.nih.gov/33792877

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání