Zobrazeno 1 - 10
of 207
pro vyhledávání: '"Yiping Qi"'
Autor:
Tingting Fan, Yanhao Cheng, Yuechao Wu, Shishi Liu, Xu Tang, Yao He, Shanyue Liao, Xuelian Zheng, Tao Zhang, Yiping Qi, Yong Zhang
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract Cytosine base editors (CBEs) and adenine base editors (ABEs) enable precise C-to-T and A-to-G edits. Recently, ABE8e, derived from TadA-8e, enhances A-to-G edits in mammalian cells and plants. Interestingly, TadA-8e can also be evolved to co
Externí odkaz:
https://doaj.org/article/4d3f41c610934d3783cf74779779f267
Autor:
Xu Tang, Ayman Eid, Rui Zhang, Yanhao Cheng, Annan Liu, Yurong Chen, Pengxu Chen, Yong Zhang, Yiping Qi
Publikováno v:
The Plant Genome, Vol 17, Iss 2, Pp n/a-n/a (2024)
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR) systems have been demonstrated to be the foremost compelling genetic tools for manipulating prokaryotic and eukaryotic genomes. Despite the robustness and versatility of
Externí odkaz:
https://doaj.org/article/5b1b9e4bdf7144fd924eb4610f4443df
Autor:
Zhaohui Zhong, Guanqing Liu, Zhongjie Tang, Shuyue Xiang, Liang Yang, Lan Huang, Yao He, Tingting Fan, Shishi Liu, Xuelian Zheng, Tao Zhang, Yiping Qi, Jian Huang, Yong Zhang
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)
Abstract Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9), sourced from a human pathogen, is the most widely used. Here, through in silico data mining, we have established an efficient plant genome engineering system usin
Externí odkaz:
https://doaj.org/article/8e7682ada5eb4553a6a8e35edf883a37
Autor:
Liyang Zhang, Gen Li, Yingxiao Zhang, Yanhao Cheng, Nathaniel Roberts, Steve E. Glenn, Diane DeZwaan-McCabe, H. Tomas Rube, Jeff Manthey, Gary Coleman, Christopher A. Vakulskas, Yiping Qi
Publikováno v:
Genome Biology, Vol 24, Iss 1, Pp 1-19 (2023)
Abstract Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has been widely used for genome editing in mammalian cells and plants due to its distinct characteristics from Cas9. Despite being one of the most robust Cas12a
Externí odkaz:
https://doaj.org/article/db0922eabb8c46788d5ef6a754bef15a
Publikováno v:
Frontiers in Genome Editing, Vol 5 (2023)
Cas12a, also known as Cpf1, is a highly versatile CRISPR-Cas enzyme that has been widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a has unique features that make it a highly efficient genome editing tool at AT-rich genomi
Externí odkaz:
https://doaj.org/article/12f5c3d81c044d169219210ee8e7107f
Publikováno v:
The Plant Genome, Vol 16, Iss 2, Pp n/a-n/a (2023)
Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) nucleases like Cas9 and Cas12a are revolutionizing plant basic research and crop breeding. A major advantage of CRISPR over earlier nucleases systems is its capability of mul
Externí odkaz:
https://doaj.org/article/425fb47ca8f64ae8a50fc600e3a1b5bf
Autor:
Yingxiao Zhang, Qiurong Ren, Xu Tang, Shishi Liu, Aimee A. Malzahn, Jianping Zhou, Jiaheng Wang, Desuo Yin, Changtian Pan, Mingzhu Yuan, Lan Huang, Han Yang, Yuxin Zhao, Qing Fang, Xuelian Zheng, Li Tian, Yanhao Cheng, Ysa Le, Bailey McCoy, Lidiya Franklin, Jeremy D. Selengut, Stephen M. Mount, Qiudeng Que, Yong Zhang, Yiping Qi
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
CRISPR-Cas12a is a promising system for targeting AT-rich regions of the genome. Here the authors identify Cas12a orthologs with expanded targeting scope and develop a highly multiplexable editing system in rice.
Externí odkaz:
https://doaj.org/article/d6eb08b3515f47118d396c6a80d7329c
Autor:
Yingxiao Zhang, Yanhao Cheng, Hong Fang, Nathaniel Roberts, Liyang Zhang, Christopher A. Vakulskas, Randall P. Niedz, James N. Culver, Yiping Qi
Publikováno v:
Frontiers in Genome Editing, Vol 4 (2022)
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome editing is a powerful approach for crop improvement. Traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration a
Externí odkaz:
https://doaj.org/article/797e722729544821a181e95739b8e02d
Autor:
Zhaohui Zhong, Shishi Liu, Xiaopei Liu, Binglin Liu, Xu Tang, Qiurong Ren, Jianping Zhou, Xuelian Zheng, Yiping Qi, Yong Zhang
Publikováno v:
Rice, Vol 13, Iss 1, Pp 1-13 (2020)
Abstract Background Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. In order to make this system routine
Externí odkaz:
https://doaj.org/article/5574553124f84f059fffcaed890b1c02
Autor:
Rajeev K. Varshney, Rutwik Barmukh, Manish Roorkiwal, Yiping Qi, Jana Kholova, Roberto Tuberosa, Matthew P. Reynolds, Francois Tardieu, Kadambot H. M. Siddique
Publikováno v:
Advanced Genetics, Vol 2, Iss 3, Pp n/a-n/a (2021)
Abstract The current pace of crop improvement is inadequate to feed the burgeoning human population by 2050. Higher, more stable, and sustainable crop production is required against a backdrop of drought stress, which causes significant losses in cro
Externí odkaz:
https://doaj.org/article/ae2fd2fa8b844328a472f5fd6bd97a99