Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Yinglai Wang"'
Publikováno v:
Proceedings of the 4th International Conference on Economic Management and Model Engineering, ICEMME 2022, November 18-20, 2022, Nanjing, China.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ead7abda2cedfe0c35b532f50663a887
https://doi.org/10.4108/eai.18-11-2022.2326846
https://doi.org/10.4108/eai.18-11-2022.2326846
Publikováno v:
Protein Expression and Purification. 15:115-120
A T7 promoter-based His 6 -tagging vector has been constructed that directs the synthesis in Escherichia coli of fusion proteins containing a stretch of six histidine residues at the N terminus. The vector allows overproduction and single-step purifi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14b195c3aa217247f701107b359748c6
https://doi.org/10.1006/prep.1998.0999
https://doi.org/10.1006/prep.1998.0999
Publikováno v:
2015 International Conference on Logistics, Informatics & Service Sciences (LISS); 2015, p1-6, 6p
Publikováno v:
Protein expression and purification. 16(2)
The in vitro T7 transcription system allows one to synthesize biochemical amounts of RNA molecules functionally equivalent or similar to those transcripts normally existing at extremely low levels in vivo. In this study we described a modified method
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccc498f119520681c75d50c63a7c5275
https://pubmed.ncbi.nlm.nih.gov/10419832
https://pubmed.ncbi.nlm.nih.gov/10419832
Publikováno v:
Science in China. Series C, Life sciences. 42(2)
The T7 promoter and the gene encoding tRNA(Leu) were synthesized chemically and cloned into plasmid pUC19, where the gene was transcribed in vitro with T7 RNA polymerase purified. It was found that spermidine had a negative effect on T7 transcription
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd1cae7dbb0bbbfb25c410fa58bf4bc5
https://pubmed.ncbi.nlm.nih.gov/18726472
https://pubmed.ncbi.nlm.nih.gov/18726472
Autor:
Yinglai Wang, Jianping Shi
Publikováno v:
30 Years' Review of China's Science and Technology (1949 – 1979) ISBN: 9789971950484
30 Years' Review of China's Science and Technology (1949 – 1979)
30 Years' Review of China's Science and Technology (1949 – 1979)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f97cefc8ae9299c59c783443a23a11f3
https://doi.org/10.1142/9789814412681_0006
https://doi.org/10.1142/9789814412681_0006
