Blebbistatin protects iPSC-CMs from hypercontraction and facilitates automated patch-clamp based electrophysiological study

Autor: Wener Li, Xiaojing Luo, Ying Ulbricht, Kaomei Guan

Publikováno v: Stem Cell Research, Vol 56, Iss , Pp 102565- (2021)

Recently, there have been great advances in cardiovascular channelopathy modeling and drug safety pharmacology using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). The automated patch-clamp (APC) technique overcomes the disadv

Externí odkaz: https://doaj.org/article/7887c497b2d548b08bc98d7f4f99e75b

Establishment of an automated patch-clamp platform for electrophysiological and pharmacological evaluation of hiPSC-CMs

Autor: Wener Li, Xiaojing Luo, Ying Ulbricht, Michael Wagner, Christopher Piorkowski, Ali El-Armouche, Kaomei Guan

Publikováno v: Stem Cell Research, Vol 41, Iss , Pp - (2019)

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have evolved into widely used and reliable cell sources for modeling cardiovascular channelopathies and for drug safety pharmacology. However, the electrophysiological and pharmac

Externí odkaz: https://doaj.org/article/05444fd556fe4e3eba4a081cd8dd1b2b

Electrophysiological abnormalities in VLCAD deficient hiPSC-cardiomyocytes can be improved by lowering accumulation of fatty acid oxidation intermediates

Autor: Frits A. Wijburg, Connie R. Bezzina, Suzan J. G. Knottnerus, Xiaojing Luo, Antonius Baartscheer, Kaomei Guan, Wener Li, Sacha Ferdinandusse, Ronald J.A. Wanders, Ying Ulbricht, Rob C. I. Wüst, Riekelt H. Houtkooper, Isabella Mengarelli, Gepke Visser, Ruben Coronel, Vincent Portero, Jeannette C. Bleeker, Lodewijk IJlst, Arie O. Verkerk

Publikováno v: International journal of molecular sciences, 21(7):2589. Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences, Vol 21, Iss 2589, p 2589 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 7

Patients with very long-chain acyl-CoA dehydrogenase deficiency (VLCADD) can present with life-threatening cardiac arrhythmias. The pathophysiological mechanism is unknown. We reprogrammed fibroblasts from one mildly and one severely affected VLCADD

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1048994c1c7de653958d78caa5e5c907
https://pure.amc.nl/en/publications/electrophysiological-abnormalities-in-vlcad-deficient-hipsccardiomyocytes-can-be-improved-by-lowering-accumulation-of-fatty-acid-oxidation-intermediates(7408927f-4407-47ac-bfe0-fd5a7be0c915).html