Zobrazeno 1 - 10
of 904
pro vyhledávání: '"Yeast display"'
Autor:
Fortunato Ferrara, Adeline Fanni, Andre A. R. Teixeira, Esteban Molina, Camila Leal-Lopes, Ashley DeAguero, Sara D’Angelo, M. Frank Erasmus, Laura Spector, Luis Antonio Rodriguez Carnero, Jianquan Li, Thomas J. Pohl, Nikolai Suslov, Klervi Desrumeaux, Conor McMahon, Sagar Kathuria, Andrew R. M. Bradbury
Publikováno v:
mAbs, Vol 16, Iss 1 (2024)
We previously described an in vitro single-chain fragment (scFv) library platform originally designed to generate antibodies with excellent developability properties. The platform design was based on the use of clinical antibodies as scaffolds into w
Externí odkaz:
https://doaj.org/article/08adf8bbba7940a8b4cc3c5378c32316
Autor:
Yuxin Hao, Jiabin Yan, Courtney Fraser, Aiping Jiang, Murali Anuganti, Roushu Zhang, Kenneth Lloyd, Joseph Jardine, Jessica Coppola, Rob Meijers, Jing Li, Timothy A. Springer
Publikováno v:
mAbs, Vol 16, Iss 1 (2024)
Integrins are cell surface receptors that mediate the interactions of cells with their surroundings and play essential roles in cell adhesion, migration, and homeostasis. Eight of the 24 integrins bind to the tripeptide Arg-Gly-Asp (RGD) motif in the
Externí odkaz:
https://doaj.org/article/cf98f9e736e742c0b512e4f1ef781a2c
Publikováno v:
Biomedicines, Vol 12, Iss 8, p 1863 (2024)
The availability of yeast surface display nanobody (Nb) libraries offers a convenient way to acquire antigen-specific nanobodies that may be useful for protein structure–function studies and/or therapeutic applications, complementary to the convent
Externí odkaz:
https://doaj.org/article/48d1d9bcf2844891870cc53429e0fb8c
Publikováno v:
Engineering Microbiology, Vol 3, Iss 4, Pp 100086- (2023)
Leech hyaluronidase (LHyal) is a hyperactive hyaluronic acid (HA) hydrolase that belongs to the hyaluronoglucuronidase family. Traditionally, LHyal is extracted from the heads of leeches, but the recent development of the Pichia pastoris recombinant
Externí odkaz:
https://doaj.org/article/844aa0104142472f909c7c9a8642a0d6
Publikováno v:
iRADIOLOGY, Vol 1, Iss 1, Pp 18-35 (2023)
Abstract Molecular imaging plays important roles in many fields, including disease diagnosis, therapeutic efficacy evaluation, intraoperative imaging guidance, drug metabolism monitoring, and patient selection for appropriate treatment. As a key comp
Externí odkaz:
https://doaj.org/article/0c613149d0b242b3a42b7488f53d669e
Autor:
Julia Harwardt, Felix Klaus Geyer, Katrin Schoenfeld, David Baumstark, Vera Molkenthin, Harald Kolmar
Publikováno v:
Antibodies, Vol 13, Iss 2, p 36 (2024)
The optimization of the affinity of monoclonal antibodies is crucial for the development of drug candidates, as it can impact the efficacy of the drug and, thus, the dose and dosing regimen, limit adverse effects, and reduce therapy costs. Here, we p
Externí odkaz:
https://doaj.org/article/b47864c5af2e4167adbb08566aff8bc0
Autor:
Hiroyuki Takeda, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, Takuya Miyakawa, Yuji Ito, Tatsuya Sawasaki
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficult
Externí odkaz:
https://doaj.org/article/a00f4a3c1cfb4e7bb5f0d140f48f362c
Autor:
Kendreze Holland, John Blazeck
Publikováno v:
Journal of Biological Engineering, Vol 16, Iss 1, Pp 1-12 (2022)
Abstract The eukaryotic yeast Saccharomyces cerevisiae is a model host utilized for whole cell biocatalytic conversions, protein evolution, and scientific inquiries into the pathogenesis of human disease. Over the past decade, the scale and pace of s
Externí odkaz:
https://doaj.org/article/15ab932079464ba19d8510df9e7a80e5
Publikováno v:
Microorganisms, Vol 12, Iss 1, p 179 (2024)
Sortase-mediated ligation (SML) is widely used for protein bioconjugation. However, the sortase used in this strategy typically recognizes only the N-terminal oligoglycine, which is absent in most natural proteins. To broaden the spectrum of substrat
Externí odkaz:
https://doaj.org/article/74bf864ddbef4b8298e9e4f9fe4edc66
Autor:
Jorge A. Lerma Romero, Christian Meyners, Andreas Christmann, Lisa M. Reinbold, Anna Charalampidou, Felix Hausch, Harald Kolmar
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
Protein dynamics have a great influence on the binding pockets of some therapeutic targets. Flexible protein binding sites can result in transient binding pocket formation which might have a negative impact on drug screening efforts. Here, we describ
Externí odkaz:
https://doaj.org/article/c24c05f623e04580bc234f8ff3bafcb8