Structural and Functional Characterization of the PaaI Thioesterase from Streptococcus pneumoniae Reveals a Dual Specificity for Phenylacetyl-CoA and Medium-chain Fatty Acyl-CoAs and a Novel CoA-induced Fit Mechanism

Autor: Yb, Khandokar, Srivastava P, Sarker S, Cm, Swarbrick, David Aragao, Cowieson N, Jk, Forwood

Publikováno v: Europe PubMed Central
CIÊNCIAVITAE

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A. PaaI thioesterases are members of the TE13 thioesterase family that catalyze the hydrolysis of thioester bonds between coenzyme A and phenylacetyl-Co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09d79f01be7570160d73e781fff0803f

Structural insights into GDP-mediated regulation of a bacterial acyl-CoA thioesterase.

Autor: Khandokar YB; From the School of Biomedical Sciences and., Srivastava P; From the School of Biomedical Sciences and., Cowieson N; the Life Sciences Division, Diamond Light Source, Didcot OX11 0DE, United Kingdom., Sarker S; the Department of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Melbourne, Victoria 3086, Australia, and., Aragao D; the Australian National Synchrotron, Melbourne, Victoria 3168, Australia., Das S; School of Animal and Veterinary Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia., Smith KM; From the School of Biomedical Sciences and., Raidal SR; School of Animal and Veterinary Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia., Forwood JK; From the School of Biomedical Sciences and jforwood@csu.edu.au.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2017 Dec 15; Vol. 292 (50), pp. 20461-20471. Date of Electronic Publication: 2017 Oct 02.

An efficient approach for recombinant expression and purification of the viral capsid protein from beak and feather disease virus (BFDV) in Escherichia coli.

Autor: Sarker S; School of Animal and Veterinary Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Boorooma Street, Wagga Wagga, New South Wales 2678, Australia. Electronic address: ssarker@csu.edu.au., Ghorashi SA; School of Animal and Veterinary Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Boorooma Street, Wagga Wagga, New South Wales 2678, Australia. Electronic address: aghorashi@csu.edu.au., Swarbrick CM; School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia. Electronic address: cswarbrick@csu.edu.au., Khandokar YB; School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia. Electronic address: ykhandokar@csu.edu.au., Himiari Z; School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia. Electronic address: zainab.himiari@gmail.com., Forwood JK; School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Boorooma Street, Wagga Wagga, New South Wales 2678, Australia. Electronic address: jforwood@csu.edu.au., Raidal SR; School of Animal and Veterinary Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2678, Australia; Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Boorooma Street, Wagga Wagga, New South Wales 2678, Australia. Electronic address: shraidal@csu.edu.au.

Publikováno v: Journal of virological methods [J Virol Methods] 2015 Apr; Vol. 215-216, pp. 1-8. Date of Electronic Publication: 2015 Feb 16.

Purification, crystallization and preliminary X-ray diffraction analysis of the N-acetyltransferase SAV0826 from Staphylococcus aureus.

Autor: Srivastava P; School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW 2678, Australia., Khandokar YB; School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW 2678, Australia., Forwood JK; School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW 2678, Australia.

Publikováno v: Acta crystallographica. Section F, Structural biology communications [Acta Crystallogr F Struct Biol Commun] 2014 Feb; Vol. 70 (Pt 2), pp. 211-4. Date of Electronic Publication: 2014 Jan 21.

Expression, purification and crystallization of acetyl-CoA hydrolase from Neisseria meningitidis.

Autor: Khandokar YB; School of Biomedical Sciences, Charles Sturt University, Boorooma Street, Wagga Wagga, New South Wales 2650, Australia., Londhe A, Patil S, Forwood JK

Publikováno v: Acta crystallographica. Section F, Structural biology and crystallization communications [Acta Crystallogr Sect F Struct Biol Cryst Commun] 2013 Nov; Vol. 69 (Pt 11), pp. 1303-6. Date of Electronic Publication: 2013 Oct 30.