Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Yasuya Fujita"'
Autor:
Hisae Takema, Yasuya Fujita, Akihiko Kondo, Satoshi Katahira, Takanori Tanino, Meguru Ito, Tsutomu Tanaka, Hideki Fukuda
Publikováno v:
Enzyme and Microbial Technology. 43:115-119
Enhancing the sugar uptake ability of the yeast Saccharomyces cerevisiae is a potentially important factor for efficient ethanol production during fermentation of lignocellulosic biomass. Here, we attempted to express a Pichia stipitis gene encoding
Publikováno v:
Applied and Environmental Microbiology. 70(9):5407-5414
Hemicellulose is one of the major forms of biomass in lignocellulose, and its essential component is xylan. We used a cell surface engineering system based on α-agglutinin to construct a Saccharomyces cerevisiae yeast strain codisplaying two types o
Autor:
Akihiko Kondo, Eiichi Satoh, Takeshi Matsumoto, Yohei Bito, Jun Koh, Hisayori Shigechi, Mitsuyoshi Ueda, Hideki Fukuda, Yasuya Fujita
Publikováno v:
Applied and Environmental Microbiology. 70(8):5037-5040
Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis α-amylase by using the C-terminal-half region
Publikováno v:
Biotechnology Progress. 20:688-691
To improve the cellulolytic activity of a yeast strain displaying endoglucanase II (EGII) from the filamentous fungus Trichoderma reesei QM9414, the genes encoding the cellulose-binding domain (CBD) of EGII, cellobiohydrolase I (CBHI) and cellobiohyd
Publikováno v:
Biochemical Engineering Journal. 18:149-153
Direct ethanol fermentation from corn starch cooked at low temperature (80 °C) was carried out using flocculent Saccharomyces cerevisiae strains co-expressing Rhizopus oryzae glucoamylase and Bacillus stearothermophilus α-amylase. The yeast strain
Publikováno v:
Applied and Environmental Microbiology. 70:1207-1212
A whole-cell biocatalyst with the ability to induce synergistic and sequential cellulose-degradation reaction was constructed through codisplay of three types of cellulolytic enzyme on the cell surface of the yeast Saccharomyces cerevisiae . When a c
Autor:
Akihiko Kondo, Shouji Takahashi, Motoo Arai, Yasushi Morikawa, Takashi Kawaguchi, Hirofumi Okada, Atsuo Tanaka, Hideki Fukuda, Mitsuyoshi Ueda, Yasuya Fujita
Publikováno v:
Applied and Environmental Microbiology. 68:5136-5141
For direct and efficient ethanol production from cellulosic materials, we constructed a novel cellulose-degrading yeast strain by genetically codisplaying two cellulolytic enzymes on the cell surface of Saccharomyces cerevisiae . By using a cell surf
Autor:
Akihiko Kondo, Hideki Fukuda, Hisayori Shigechi, Mitsuyoshi Ueda, Atsuo Tanaka, Ken Uyama, Takeshi Matsumoto, Yasuya Fujita
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 17:179-187
To develop novel yeasts with high starch-to-ethanol productivity, we constructed two cell-surface-engineered flocculent Saccharomyces cerevisiae strains; one co-displaying glucoamylase and α-amylase on the cell surface and the other displaying gluco
Autor:
Akihiko Kondo, Yasushi Morikawa, Satoshi Katahira, Atsuo Tanaka, Mitsuyoshi Ueda, Hirofumi Okada, Hideki Fukuda, Yasuya Fujita
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 17:189-195
We constructed a yeast-based whole-cell biocatalyst displaying Trichoderma reesei xylanase II (XYNII) on the cell-surface and endowed the yeast-cells with the ability to degrade xylan. The fusion gene encoding the mature region of XYNII and the C-ter
Autor:
Hirofumi Okada, Jin-Min Nam, Mitsuyoshi Ueda, Akihiko Kondo, Atsuo Tanaka, Yasushi Morikawa, Terukazu Arai, Yasuya Fujita
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 17:197-202
The genes encoding cellulose binding domain (CBD) from cellobiohydrolase I (CBHI) and cellobiohydrolase II (CBHII) of the filamentous fungus Trichoderma reesei were expressed on the cell surface of the yeast Saccharomyces cerevisiae by cell surface e