Diverged and Active Partitiviruses in Lichen

Autor: Syun-ichi Urayama, Nobutaka Doi, Fumie Kondo, Yuto Chiba, Yoshihiro Takaki, Miho Hirai, Yasutaka Minegishi, Daisuke Hagiwara, Takuro Nunoura

Publikováno v: Frontiers in Microbiology, Vol 11 (2020)

The lichen is a microbial consortium that mainly consists of fungi and either algae (Viridiplantae) or cyanobacteria. This structure also contains other bacteria, fungi, and viruses. However, RNA virus diversity associated with lichens is still unkno

Externí odkaz: https://doaj.org/article/6d5ab829a9cf481796edd4948b921312

Development of a Novel Detection Method Targeting an Ultrashort 25 bp Sequence Found in Agrobacterium-Mediated Transformed GM Plants

Autor: Kosuke Nakamura, Junichi Mano, Mari Onishi, Yasutaka Minegishi, Kazunari Kondo, Satoshi Futo, Kazumi Kitta, Keisuke Soga, Reona Takabatake

Publikováno v: Journal of Agricultural and Food Chemistry. 68:15327-15334

Agrobacterium-mediated transformation is the most commonly used technique for plant genetic engineering. During the transformation, a T-DNA region, which is flanked by the right border (RB) and the left border, is transferred to plant nuclear chromos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b47eb5c0906b48ced1f311b8a91b4d3a
https://doi.org/10.1021/acs.jafc.0c03864

A Novel Real-Time PCR-Based Screening Test with Pooled Fecal Samples for Bovine Johne’s Disease

Autor: Yasuyuki Mori, Reiko Nagata, Yasutaka Minegishi, Shingo Kishizuka, Satoko Kawaji, Akiko Mita, Yumi Saruyama, Masahiro Saito

Publikováno v: J Clin Microbiol

Johne’s disease (JD) is an economically important infectious disease in livestock farming caused by Mycobacterium avium subsp. paratuberculosis. As an alternative to serological tests, which are used mainly for the screening of whole herds, we deve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05de3593d47b97710ec60f8abe81c5dc
https://doi.org/10.1128/jcm.01761-20

Development and evaluation of rapid screening detection methods for genetically modified crops using loop-mediated isothermal amplification

Autor: Kazunari Kondo, Kazumi Kitta, Junichi Mano, Akio Noguchi, Yukari Kagiya, Reona Takabatake, Satoshi Futo, Sabina Yeasmin, Yasutaka Minegishi

Publikováno v: SC30201803130001
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We developed new loop-mediated isothermal amplification (LAMP)-based detection methods for the screening of genetically modified (GM) maize and soybean events. The LAMP methods developed targeted seven sequences: cauliflower mosaic virus 35S promoter

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49abebe24bb10069906c1104d9b37615
https://doi.org/10.1016/j.foodchem.2017.12.036

Inter-Laboratory Study to Validate New Rapid Screening Methods for Kudoa septempunctata

Autor: Koji Makitsuru, Shunichi Inagaki, Mari Sasaki, Natsumi Nojima, Maiko Kawabe, Ogasawara Kunitoshi, Kunihiko Nakane, Yasutaka Minegishi, Hiroaki Tsuchioka, Kazumi Horikawa, Takahiro Ohnishi, Bong-Soo Lim, Yoshiko Sugita-Konishi, Nobuo Miyazaki

Publikováno v: Biocontrol Science. 21:135-138

Kudoa septempunctata is the causative agent of a food-borne disease associated with the ingestion of raw olive flounder. As the current qRT-PCR method for its detection is time-consuming, a rapid and simple method is required. Recently, a new real-ti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d427342633d13787f642bbcfb80ad121
https://doi.org/10.4265/bio.21.135