Assembly of the phagocyte NADPH oxidase complex: chimeric constructs derived from the cytosolic components as tools for exploring structure-function relationships

Autor: Yevgeny Berdichevsky, Shahar Molshanski-Mor, Yara Gorzalczany, Iris Dahan, Miriam Hirshberg, Nathalie Alloul, Rive Sarfstein, Yael Nakash, Yelena Ugolev, Edgar Pick, Ariel Mizrahi

Publikováno v: Journal of Leukocyte Biology. 79:881-895

Phagocytes generate superoxide (O2.−) by an enzyme complex known as reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Its catalytic component, responsible for the NADPH-driven reduction of oxygen to O2.−, is flavocytochrome b55

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fca9a12c1f47022e61e08e3b76ce9c26
https://doi.org/10.1189/jlb.1005553

The Guanine Nucleotide Exchange Factor Trio Activates the Phagocyte NADPH Oxidase in the Absence of GDP to GTP Exchange on Rac

Autor: Carolyn Weinbaum, Rive Sarfstein, Yara Gorzalczany, Edgar Pick, Natalia Sigal, Yi Zheng

Publikováno v: Journal of Biological Chemistry. 278:4854-4861

The superoxide-generating NADPH oxidase complex of phagocytes consists of a membrane-associated flavocytochrome b(559) and four cytosolic components as follows: p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Activation of the oxi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fdda526f1d448b1fc9d1571978ea89e7
https://doi.org/10.1074/jbc.m211011200

A Prenylated p67 -Rac1 Chimera Elicits NADPH-dependent Superoxide Production by Phagocyte Membranes in the Absence of an Activator and of p47

Autor: Nathalie Alloul, Natalia Sigal, Yara Gorzalczany, Edgar Pick, Carolyn Weinbaum

Publikováno v: Journal of Biological Chemistry. 277:18605-18610

Activation of the superoxide-generating NADPH oxidase of phagocytes is the result of the assembly of a membrane-localized flavocytochrome (cytochromeb 559) with the cytosolic components p47phox, p67phox , and the small GTPase Rac. Activation can be r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7246c66ea6f781b150208ef4562f4da2
https://doi.org/10.1074/jbc.m202114200

Activation of the Superoxide-Generating NADPH Oxidase by Chimeric Proteins Consisting of Segments of the Cytosolic Component p67phoxand the Small GTPase Rac1

Autor: Natalia Sigal, Nathalie Alloul, Yara Gorzalczany, Michal Itan, Edgar Pick

Publikováno v: Biochemistry. 40:14557-14566

Activation of the superoxide (O2(-))-generating NADPH oxidase of phagocytes is the consequence of the assembly of a membrane-associated flavocytochrome b(559) with the cytosolic proteins p47(phox) and p67(phox) and the small GTPase Rac (1 or 2). We p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26122bea7730e6e08334ad56bf7b6e24
https://doi.org/10.1021/bi0117347

Dual role of Rac in the assembly of NADPH oxidase, tethering to the membrane and activation of p67phox: a study based on mutagenesis of p67phox-Rac1 chimeras

Autor: Rive, Sarfstein, Yara, Gorzalczany, Ariel, Mizrahi, Yevgeny, Berdichevsky, Shahar, Molshanski-Mor, Carolyn, Weinbaum, Miriam, Hirshberg, Marie-Claire, Dagher, Edgar, Pick

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, 2004, 279 (16), pp.16007-16. ⟨10.1074/jbc.M312394200⟩
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279 (16), pp.16007-16. ⟨10.1074/jbc.M312394200⟩

International audience; NADPH oxidase activation involves the assembly of membrane-localized cytochrome b559 with the cytosolic components p47phox, p67phox, and the small GTPase Rac. Assembly is mimicked by a cell-free system consisting of membranes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::8bc33158d830560a31588ec8e51e015f
https://hal.science/hal-00820746