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Akademický článek

Characterization of two bacterial multi-flavinylated proteins harboring multiple covalent flavin cofactors

Autor: Yapei Tong, Henriette J. Rozeboom, Marnix R. Loonstra, Hein J. Wijma, Marco W. Fraaije

Publikováno v: BBA Advances, Vol 4, Iss , Pp 100097- (2023)

In recent years, studies have shown that a large number of bacteria secrete multi-flavinylated proteins. The exact roles and properties, of these extracellular flavoproteins that contain multiple covalently anchored FMN cofactors, are still largely u

Externí odkaz: https://doaj.org/article/47bacea9a7bd4e00836afb0201bd2f67

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Flavin-tag: exploiting a flavin transferase for protein labeling and engineering

Autor: Yapei Tong

Site-specific protein labeling plays a core role in investigating protein function at molecular level. A multitude of protein labelling techniques has been developed which are based on chemical and/or enzymatic methods. Enzyme-based labeling of prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df7a56be62085be1476368e176ad825d
https://research.rug.nl/en/publications/c6b8f56e-35a4-42ec-8a8a-21ba5205eb5a

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Flavin-Tag

Autor: Jeroen Drenth, Misun Lee, Yapei Tong, Marco W. Fraaije

Publikováno v: BIOCONJUGATE CHEMISTRY, 32(8), 1559-1563. AMER CHEMICAL SOC

Site-specific protein labeling methods are highly valuable tools for research and applications. We present a new protein labeling method that allows covalent attachment of a chromo-and fluorogenic flavin (FMN) to any targeted protein using a short fl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d4952611d4cfe75043853db2d61b34b
https://doi.org/10.1021/acs.bioconjchem.1c00306

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Broadening the Scope of the Flavin‐Tag Method by Improving Flavin Incorporation and Incorporating Flavin Analogs

Autor: Yapei Tong, Marnix R. Loonstra, Marco W. Fraaije

Publikováno v: ChemBioChem, 23(11):e202200144. WILEY-V C H VERLAG GMBH

Methods for facile site-selective modifications of proteins are in high demand. We have recently shown that a flavin transferase can be used for site-specific covalent attachment of a chromo- and fluorogenic flavin (FMN) to any targeted protein. Alth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c20e8f61f6827a8a66a30c1e9a4bf1a1
https://doi.org/10.1002/cbic.202200144

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Substrate binding tunes the reactivity of hispidin 3-hydroxylase, a flavoprotein monooxygenase involved in fungal bioluminescence

Autor: Marco W. Fraaije, Yapei Tong, Willem J. H. van Berkel, Milos Trajkovic, Simone Savino

Publikováno v: The Journal of Biological Chemistry, 295(47), 16013-16022. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
J Biol Chem
Journal of Biological Chemistry 295 (2020) 47
Journal of Biological Chemistry, 295(47), 16013-16022

Fungal bioluminescence was recently shown to depend on a unique oxygen-dependent system of several enzymes. However, the identities of the enzymes did not reveal the full biochemical details of this process, as the enzymes do not bear resemblance to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b42915ad2c9ff76579956aeb7c8fa84e
https://research.rug.nl/en/publications/741c2e3d-0420-468b-b3a4-eb4d15024d8a

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Akademický článek

Substrate binding tunes the reactivity of hispidin 3-hydroxylase, a flavoprotein monooxygenase involved in fungal bioluminescence.

Autor: Yapei Tong¹, Trajkovic, Milos¹, Savino, Simone¹, van Berkel, Willem J. H.², Fraaije, Marco W.¹ m.w.fraaije@rug.nl

Publikováno v: Journal of Biological Chemistry. 11/20/2020, Vol. 295 Issue 47, p16013-16022. 10p.

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