Zobrazeno 1 - 10
of 265
pro vyhledávání: '"Yap, Miranda"'
Mammalian cell culture is typically operated at the physiological temperature of 37°C. Low temperature cell culture at 30-33°C, in particular for CHO cells, increased the specific productivity of many recombinant proteins amongst many other benefit
Externí odkaz:
http://hdl.handle.net/1721.1/30382
When grown under hypothermic conditions, Chinese Hamster Ovary (CHO) cells become growth arrested in the G₀/G₁ phase of the cell cycle and also often exhibit increased recombinant protein production. In this study, we have validated this hypothes
Externí odkaz:
http://hdl.handle.net/1721.1/30379
In the field of biologics production, productivity and stability of the transfected gene of interest are two very important attributes that dictate if a production process is viable. To further understand and improve these two traits, we would need t
Externí odkaz:
http://hdl.handle.net/1721.1/7504
Most glyco-engineering approaches used to improve quality of recombinant glycoproteins involve the manipulation of glycosyltransferase and/or glycosidase expression. We investigated whether the over expression of nucleotide sugar transporters, partic
Externí odkaz:
http://hdl.handle.net/1721.1/7487
Autor:
Chu, Jhih-Wei, Yin, Jin, Mazyar, Oleg, Goh, Lin-Tang, Yap, Miranda G.S., Wang, Daniel I.C., Trout, Bernhardt L.
We present results of molecular simulations, quantum mechanical calculations, and experimental data aimed towards the rational design of solvent formulations. In particular, we have found that the rate limitation of oxidation of methionine groups is
Externí odkaz:
http://hdl.handle.net/1721.1/3794
The production of recombinant glycoproteins from mammalian cell cultures requires robust processes that can achieve high protein yield while ensuring the efficacy of these proteins as human therapeutics. We describe two approaches currently being dev
Externí odkaz:
http://hdl.handle.net/1721.1/3781
The bio-manufacturing of recombinant proteins from mammalian cell cultures requires robust processes that can maximize protein yield while ensuring the efficacy of these proteins as human therapeutics. Recognizing that the challenge of improving prot
Externí odkaz:
http://hdl.handle.net/1721.1/3782
Autor:
Luo, Biao, Meng-Ling, Choong, Heard, Amanda, Li, Zhe, Moore, Kateri, Kaiser, Chris, Lemischka, Ihor R., Yap, Miranda G.S., Lodish, Harvey F.
Multipotent hematopoietic stem cells (HSCs) are progenitors of all types of hematopoietic cells, and the efficient isolation and propagation of HSCs will significantly enhance our ability to manage many human disorders with bone marrow transplantatio
Externí odkaz:
http://hdl.handle.net/1721.1/4044
Autor:
Yusufi, Faraaz Noor Khan, Lakshmanan, Meiyappan, Ho, Ying Swan, Loo, Bernard Liat Wen, Ariyaratne, Pramila, Yang, Yuansheng, Ng, Say Kong, Tan, Tessa Rui Min, Yeo, Hock Chuan, Lim, Hsueh Lee, Ng, Sze Wai, Hiu, Ai Ping, Chow, Chung Ping, Wan, Corrine, Chen, Shuwen, Teo, Gavin, Song, Gao, Chin, Ju Xin, Ruan, Xiaoan, Sung, Ken Wing Kin, Hu, Wei-Shou, Yap, Miranda Gek Sim, Bardor, Muriel, Nagarajan, Niranjan, Lee, Dong-Yup
Publikováno v:
In Cell Systems 24 May 2017 4(5):530-542
Autor:
Courtes, Franck C., Vardy, Leah, Wong, Niki S.C., Bardor, Muriel, Yap, Miranda G.S., Lee, Dong-Yup
Publikováno v:
In New BIOTECHNOLOGY 25 September 2014 31(5):514-523