Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Yamal Al-Ramahi"'
Autor:
Yamal Al-ramahi, Akos Nyerges, Yago Margolles, Lidia Cerdán, Gyorgyi Ferenc, Csaba Pál, Luis Ángel Fernández, Víctor de Lorenzo
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
Yamal Al-ramahi et al. describe an accelerated laboratory evolution method that applies ssDNA recombineering and bacterial surface display to engineer nanobody epitope recognition. As a proof-of-concept, they evolved TD4 recognition to bind the trans
Externí odkaz:
https://doaj.org/article/7cfb63c37a134a1381dcf213cbee4ff5
Autor:
Elena, Velázquez, Yamal, Al-Ramahi, Jonathan, Tellechea-Luzardo, Natalio, Krasnogor, Víctor, de Lorenzo
Publikováno v:
ACS Synthetic Biology
Genome editing methods based on group II introns (known as targetron technology) have long been used as a gene knockout strategy in a wide range of organisms, in a fashion independent of homologous recombination. Yet, their utility as delivery system
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e25e51ed1a178afa310ec2b0942fc154
https://pubmed.ncbi.nlm.nih.gov/34601868
https://pubmed.ncbi.nlm.nih.gov/34601868
Publikováno v:
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
ACS Synthetic Biology
Digital.CSIC. Repositorio Institucional del CSIC
instname
Consejo Superior de Investigaciones Científicas (CSIC)
ACS Synthetic Biology
Digital.CSIC. Repositorio Institucional del CSIC
instname
Group II introns were developed some time ago as tools for the construction of knockout mutants in a wide range of organisms, ranging from Gram-positive and Gram-negative bacteria to human cells. Utilizing these introns is advantageous because they a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0947f40900ee1da43b6b73cce3dea47c
https://doi.org/10.1021/acssynbio.9b00293
https://doi.org/10.1021/acssynbio.9b00293
Autor:
Víctor de Lorenzo, Yamal Al-Ramahi, Ákos Nyerges, Györgyi Ferenc, Lidia Cerdán, Csaba Pál, Yago Margolles, Luis Ángel Fernández
Publikováno v:
Communications Biology
Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
ssDNA recombineering has been exploited to hyperdiversify genomically-encoded nanobodies displayed on the surface of Escherichia coli for originating new binding properties. As a proof-of-principle a nanobody recognizing the antigen TirM from enteroh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efc60e7281db791cbbba1af9a36076f0
https://doi.org/10.1101/2021.01.28.428624
https://doi.org/10.1101/2021.01.28.428624
Autor:
Alejandro Acosta, Ana Suárez-Suárez, Yamal Al-Ramahi, Olga Genilloud, Juan L. Ramos, Jennifer Solano, Pablo Yarza, Carmen Gómez Guerrero, Nieves López-Cortés, Manuel Ferrer, Eduardo Santero, Virginia Martínez, Michael Richter, Fernando Rojo, Julián Pérez-Pérez, María-Eugenia Guazzaroni, Laura I. de Eugenio, María Alcaide, Ramon Rosselló-Móra, Silvia Marqués, María Antonia Molina-Henares, Adela García-Salamanca, Pieter van Dillewijn
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
instname
instname
The phylogenetic and functional structure of the microbial community residing in a Ca2+-rich anoxic sediment of a sub-saline shallow lake (Laguna de Carrizo, initially operated as a gypsum (CaSO4 × 2 H2O) mine) was estimated by analyzing the diversi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cda299e2b9e377fa1637db5c86773d8
http://hdl.handle.net/10261/129033
http://hdl.handle.net/10261/129033
Autor:
Ana Beloqui, María Eugenia Guazzaroni, Florencio Pazos, José M. Vieites, Marta Godoy, Olga V. Golyshina, Tatyana N. Chernikova, Agnes Waliczek, Rafael Silva-Rocha, Yamal Al-Ramahi, Violetta La Cono, Carmen Mendez, José A. Salas, Roberto Solano, Michail M. Yakimov, Kenneth N. Timmis, Peter N. Golyshin, Manuel Ferrer
Publikováno v:
Science. 330:912-912
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::537852a081b381996a58dcca21d44edc
https://doi.org/10.1126/science.330.6006.912-a
https://doi.org/10.1126/science.330.6006.912-a
Publikováno v:
ChemBioChem
Digital.CSIC. Repositorio Institucional del CSIC
instname
Digital.CSIC. Repositorio Institucional del CSIC
instname
A variant of the soil bacterium Pseudomonas putida with a genome containing a ∼20 % replacement of the whole of thymine (T) by uracil (U) was made by deleting genes ung (uracil DNA glycosylase) and dut (deoxyuridine 5′‐triphosphate nucleotide h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0afa9c3d65d2dff59694fa4a8e0e0b79
Publikováno v:
Current Protocols
Targetron technology, a gene-editing approach based on the use of mobile group II introns, is particularly useful for bacterial strains deficient in homologous recombination. Specifically, the Ll.LtrB intron from Lactococcus lactis can be used in a w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04219dd9da2a2b0493034d9acc3da3a3