Identification of amino acid residues of mammalian mitochondrial phosphate carrier important for its functional expression in yeast cells, as achieved by PCR-mediated random mutation and gap-repair cloning

Autor: Yamagoshi, Ryohei, Yamamoto, Takenori, Hashimoto, Mitsuru, Sugahara, Ryohei, Shiotsuki, Takahiro, Miyoshi, Hideto, Terada, Hiroshi, Shinohara, Yasuo

Publikováno v: In Mitochondrion January 2017 32:1-9

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Immunoblotting with Peptide Antibodies: Differential Immunoreactivities Caused by Certain Amino Acid Substitutions in a Short Peptide and Possible Effects of Differential Refolding of the Peptide on a Nitrocellulose or PVDF Membrane.

Autor: Yamamoto T; Institute for Genome Research, University of Tokushima, Kuramotocho 3-18, Tokushima, 770-8503, Japan. tyamamo@genome.tokushima-u.ac.jp.; Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima, Japan. tyamamo@genome.tokushima-u.ac.jp., Matsuo T; Institute for Genome Research, University of Tokushima, Kuramotocho 3-18, Tokushima, 770-8503, Japan.; Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima, Japan., Yamamoto A; Faculty of Pharmaceutical Science, Suzuka University of Medical Science, Suzuka, Japan., Yamagoshi R; Institute for Genome Research, University of Tokushima, Kuramotocho 3-18, Tokushima, 770-8503, Japan.; Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima, Japan., Ohkura K; Faculty of Pharmaceutical Science, Suzuka University of Medical Science, Suzuka, Japan., Kataoka M; Biomarker Analysis Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan., Shinohara Y; Institute for Genome Research, University of Tokushima, Kuramotocho 3-18, Tokushima, 770-8503, Japan.; Faculty of Pharmaceutical Sciences, University of Tokushima, Tokushima, Japan.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1348, pp. 303-10.