Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Yale S Michaels"'
Autor:
Laura Prochazka, Yale S Michaels, Charles Lau, Ross D Jones, Mona Siu, Ting Yin, Diana Wu, Esther Jang, Mercedes Vázquez‐Cantú, Penney M Gilbert, Himanshu Kaul, Yaakov Benenson, Peter W Zandstra
Publikováno v:
Molecular Systems Biology, Vol 18, Iss 11, Pp 1-22 (2022)
Abstract During development, cell state transitions are coordinated through changes in the identity of molecular regulators in a cell type‐ and dose‐specific manner. The ability to rationally engineer such transitions in human pluripotent stem ce
Externí odkaz:
https://doaj.org/article/e2b62297353c4d7b84600c9edd7e25df
Publikováno v:
npj Regenerative Medicine, Vol 7, Iss 1, Pp 1-13 (2022)
Abstract The generation of T-cells from stem cells in vitro could provide an alternative source of cells for immunotherapies. T-cell development from hematopoietic stem and progenitor cells (HSPCs) is tightly regulated through Notch pathway activatio
Externí odkaz:
https://doaj.org/article/71cda5e4d6d64e01a7fc4d2753c6d8d5
Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
Autor:
David J. H. F. Knapp, Yale S. Michaels, Max Jamilly, Quentin R. V. Ferry, Hector Barbosa, Thomas A. Milne, Tudor A. Fulga
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.
Externí odkaz:
https://doaj.org/article/088592c408394975893eeb5eb3ce5546
Autor:
Yale S. Michaels, Mike B. Barnkob, Hector Barbosa, Toni A. Baeumler, Mary K. Thompson, Violaine Andre, Huw Colin-York, Marco Fritzsche, Uzi Gileadi, Hilary M. Sheppard, David J. H. F. Knapp, Thomas A. Milne, Vincenzo Cerundolo, Tudor A. Fulga
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Analogue regulation of gene expression is important for normal function in mammals but existing genetic technologies are designed to achieve ON/OFF control. Here the authors develop synthetic microRNA silencing-mediated fine-tuners (miSFITs) to preci
Externí odkaz:
https://doaj.org/article/f84f595575f647518a682f40b466a3e0
Publikováno v:
GEN Biotechnology. 2:106-119
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2dba316444ce3be65c1d64a55d1b6f40
https://doi.org/10.1089/genbio.2023.0008
https://doi.org/10.1089/genbio.2023.0008
Publikováno v:
Frontiers in Immunology, Vol 11 (2020)
Chimeric antigen receptor (CAR) T cells have emerged as a promising treatment for patients with advanced B-cell cancers. However, widespread application of the therapy is currently limited by potentially life-threatening toxicities due to a lack of c
Externí odkaz:
https://doaj.org/article/6201bcd6798b4d0bbe1f9f82ba48b9f2
Autor:
Yale S. Michaels, Mike B. Barnkob, Hector Barbosa, Toni A. Baeumler, Mary K. Thompson, Violaine Andre, Huw Colin-York, Marco Fritzsche, Uzi Gileadi, Hilary M. Sheppard, David J. H. F. Knapp, Thomas A. Milne, Vincenzo Cerundolo, Tudor A. Fulga
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-3 (2019)
Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other constr
Externí odkaz:
https://doaj.org/article/f982718f87894f9b9dd74cb5a98758c7
Autor:
Qianxin Wu, Quentin R. V. Ferry, Toni A. Baeumler, Yale S. Michaels, Dimitrios M. Vitsios, Omer Habib, Roland Arnold, Xiaowei Jiang, Stefano Maio, Bruno R. Steinkraus, Marta Tapia, Paolo Piazza, Ni Xu, Georg A. Holländer, Thomas A. Milne, Jin-Soo Kim, Anton J. Enright, Andrew R. Bassett, Tudor A. Fulga
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-17 (2017)
RNA regulatory elements (RREs) are important post-transcriptional control features but studying them requires disrupting their activity without disturbing cellular homeostasis. Here the authors present GenERA, a CRISPR-Cas9 screening platform of in s
Externí odkaz:
https://doaj.org/article/db4eb21f42e2466d9fc7c9b4e4aa2f37
Publikováno v:
Methods in Molecular Biology ISBN: 9781071629819
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0ef40b6456ecc9649f1081e1b4cec344
https://doi.org/10.1007/978-1-0716-2982-6_16
https://doi.org/10.1007/978-1-0716-2982-6_16
Autor:
Yale S. Michaels, John M. Edgar, Matthew C. Major, Elizabeth L. Castle, Carla Zimmerman, Ting Yin, Andrew Hagner, Charles Lau, Han Hsuan Hsu, M. Iliana Ibañez-Rios, Lauren J. Durland, David J. H. F. Knapp, Peter W. Zandstra
Publikováno v:
Science Advances. 8
T cells show tremendous efficacy as cellular therapeutics. However, obtaining primary T cells from human donors is expensive and variable. Pluripotent stem cells (PSCs) have the potential to provide a renewable source of T cells, but differentiating
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc04440b7a020a348127528a8b9d7eff
https://doi.org/10.1126/sciadv.abn5522
https://doi.org/10.1126/sciadv.abn5522